大鼠背部气囊滑膜炎模型的探讨

发布时间：2019-01-01 10:45

【摘要】：目的:探讨不同致炎剂致大鼠背部气囊滑膜炎模型的特点。方法:实验第1d和第3d大鼠皮下注射空气20ml;第6d,大鼠皮下注射空气10ml,然后向气囊内注入不同致炎剂———角叉菜胶(Car)和脂多糖(LPS),观察不同浓度各致炎剂作用大鼠背部皮下气囊炎症发生、发展情况及对炎症因子的影响。结果:Car各剂量致炎组均可促进大鼠背部气囊滑膜炎炎性液的渗出,促进渗出液中白细胞聚集,升高渗出液总蛋白、NO、MDA和PGE2的含量,升高血清MDA含量和降低血清SOD活力,实验剂量Car100mg/kg、150mg/kg下,与空白对照组相比具有显著性差异。LPS致炎组在实验剂量下,对大鼠气囊滑膜炎炎性渗出液的渗出量、渗出液中MDA含量和血清SOD活力与空白对照组相比差异不显著,但对渗出液中总蛋白、NO、MDA和PGE2的含量的影响,随致炎剂量的增加,呈现出先升高后降低的趋势。结论:向大鼠皮下气囊注入角叉菜胶或脂多糖均可成功建立大鼠背部气囊滑膜炎模型。综合比较提示,Car(100mg/kg)大鼠背部气囊滑膜炎模型能较好地模拟在单一致炎因子存在情况下引起的炎症状态。
[Abstract]:Objective: to investigate the characteristics of rat models of dorsal airbag synovitis induced by different inflammatory agents. Methods: air 20 ml was injected subcutaneously into the rats on the 1st and 3rd day of the experiment. On the 6th day, the rats were injected subcutaneously with air 10 ml, and then were injected with different inflammatory agents, carrageenin (Car) and lipopolysaccharide (LPS), to observe the occurrence of subcutaneous inflammation in the back of rats. Development and effects on inflammatory factors. Results: each dose of Car induced inflammation could promote the exudation of inflammatory fluid of rat back airbag synovitis, promote leukocyte aggregation in exudate, increase the contents of total protein, NO,MDA and PGE2 in exudate, increase the content of serum MDA and decrease the activity of serum SOD. The experimental dose of Car100mg/kg,150mg/kg was significantly different from that of the blank control group. The amount of inflammatory exudate in LPS induced inflammation group was increased in rats with synovitis. There was no significant difference in the content of MDA and the activity of serum SOD between the exudate and the control group, but the effect on the contents of total protein, NO,MDA and PGE2 in the exudate showed a tendency of first increasing and then decreasing with the increase of the dose of inflammation. Conclusion: carrageenin or lipopolysaccharide can be injected subcutaneously into the rat airbag to establish the rat model of dorsal airbag synovitis. The comprehensive comparison indicated that the model of dorsal airbag synovitis in, Car (100mg/kg rats could well simulate the inflammatory state caused by the presence of single inflammatory factor.
【作者单位】：北京中医药大学中药学院;北京市药品检验所;
【分类号】：R-332

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