中介素减轻大鼠高肺血流性肺血管结构重构

发布时间：2019-02-26 10:29

【摘要】：目的研究中介素(IMD)对大鼠高肺血流性肺血管结构重构的调节作用及其机制。方法雄性SD大鼠21只,随机分为对照组(n=7),分流组(n=8)和分流+IMD组(n=6)。对后两组大鼠行腹主动脉-下腔静脉分流术。8周后,对分流+IMD组大鼠,皮下埋微量渗透泵持续予IMD 1.5μg/(kg·h)。继续饲养2周后测定动脉平均压(mPAP)和右心室/(左心室+室间隔)重量比值,检测肺血管形态学变化,硝酸酶还原法测定血清和肺组织匀浆一氧化氮(NO)含量,Western blot方法测定肺组织内皮型一氧化氮合酶(eNOS)含量。结果与对照组相比,分流组大鼠mPAP、右心室肥厚程度、肺小血管肌化程度以及肺中、小动脉相对中膜厚度均明显增加,血清和肺组织匀浆NO含量明显降低,肺组织eNOS蛋白含量降低。IMD使肺动脉压力明显回降,右心室肥厚程度减轻,肺血管结构改变缓解,血清和肺组织匀浆NO水平以及肺组织eNOS蛋白含量均明显升高。结论 IMD可能通过eNOS/NO途径,减轻高肺血流性肺动脉高压和肺血管结构重构的形成。
[Abstract]:Objective to study the regulatory effect and mechanism of interleukin-(IMD) (IL-1) on pulmonary vascular structural remodeling in rats with high pulmonary blood flow. Methods Twenty-one male SD rats were randomly divided into three groups: control group (n = 7), shunt group (n = 8) and shunting IMD group (n = 6). After 8 weeks, IMD 1.5 渭 g / (kg 路h).) was subcutaneously embedded into the subcutaneous osmotic pump of IMD rats in the latter two groups, and the rats in the shunts group were treated with IMD 1.5 渭 g / (kg 路h).) for 8 weeks. After 2 weeks of feeding, the mean arterial pressure (mPAP) and the ratio of right ventricular / (left ventricular septum) weight were measured, and the morphological changes of pulmonary vessels were detected. The nitric oxide (NO) content in serum and lung homogenate was measured by nitrate reductase method. The content of endothelial nitric oxide synthase (eNOS) in lung tissue was determined by Western blot. Results compared with the control group, the degree of mPAP, right ventricular hypertrophy, the degree of pulmonary small vessel myosis and the relative media thickness of pulmonary arterioles in shunt group were significantly increased, while the content of NO in serum and lung homogenate was significantly lower than that in control group. The pulmonary artery pressure, right ventricular hypertrophy, pulmonary vascular structure change, serum and lung homogenate NO level and lung eNOS protein content were significantly increased. The eNOS protein content in the lung tissue was decreased, the right ventricular hypertrophy was alleviated, and the serum and lung homogenate NO levels were significantly increased as compared with the control group (P < 0.05). Conclusion IMD may reduce the formation of pulmonary hypertension and pulmonary vascular structural remodeling through eNOS/NO pathway.
【作者单位】：北京大学第一医院儿科;
【基金】：国家自然科学基金(30973226)
【分类号】：R363

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