产前缺氧程序控制6周龄兔颈动脉内皮依赖性舒张功能及其相关分子机制

发布时间：2019-03-16 16:15

【摘要】：目的研究产前缺氧对6周龄新西兰兔颈动脉内皮依赖性舒张功能的影响及相关分子机制。方法同期怀孕的新西兰母兔随机分为常氧对照组(n=5)和缺氧组(12%O2,n=5),于妊娠第10天开始相应的干预,至妊娠第29天结束,自然分娩。每窝随机留1只雄性新生兔饲养,共得10只雄性新生兔,母乳喂养至6周。检测6周龄兔离体颈动脉内皮依赖性舒张功能;利用同位素方法测定内皮型一氧化氮合酶活性;利用Western blot、免疫组织化学法检测颈动脉内皮型一氧化氮合酶、热休克蛋白90表达。结果产前缺氧损伤6周龄兔颈动脉内皮依赖性舒张功能,降低内皮型一氧化氮合酶活性,下调热休克蛋白90表达(P0.05)。结论产前缺氧通过下调6周龄兔颈动脉热休克蛋白90表达,降低内皮型一氧化氮合酶活性,从而损伤颈动脉内皮依赖性舒张功能。
[Abstract]:Objective to study the effect of prenatal hypoxia on endothelium-dependent vasodilation of carotid artery in 6-week-old New Zealand rabbits and its molecular mechanism. Methods New Zealand female rabbits who were pregnant at the same time were randomly divided into normoxic control group (n = 5) and hypoxia group (n = 12% O _ 2, n = 5). The intervention began on the 10th day of pregnancy and ended on the 29th day of gestation. One male newborn rabbit was kept at random in each litter, and 10 male newborn rabbits were breastfed for 6 weeks. The endothelium-dependent vasodilation of carotid artery in 6-week-old rabbits was measured, and the activity of endothelial nitric oxide synthase (Enos) was measured by isotope method. The expression of endothelial nitric oxide synthase (Enos) and heat shock protein 90 (HSP90) in carotid artery were detected by Western blot, immunohistochemical method. Results the endothelium-dependent vasodilation of carotid artery decreased the activity of endothelial nitric oxide synthase and down-regulated the expression of heat shock protein-90 (HSP90) in 6-week-old rabbits with prenatal hypoxia injury (P0.05). Conclusion Prenatal hypoxia can reduce the activity of endothelial nitric oxide synthase by down-regulating the expression of heat shock protein-90 (HSP90) in the carotid artery of 6-week-old rabbits and thus impair the endothelium-dependent dilation function of carotid artery.
【作者单位】：福建省泉州医学高等专科学校;福建医科大学附属第二医院;
【基金】：福建省教育厅项目(JA11322) 泉州市科委重点项目(2012Z72)
【分类号】：R363

【参考文献】