γ-氨基丁酸对情绪应激大鼠血脂的影响

发布时间：2019-03-23 18:02

【摘要】：目的探讨γ-氨基丁酸(GABA)对情绪应激大鼠血脂代谢的调节作用。方法利用不确定性空瓶刺激的方法建立焦虑应激大鼠模型,将40只雄性SD大鼠随机分为对照组、模型组、GABA 0.5(L)、1.0(M)和2.0 mg/kg(H)组,应激前分别给予大鼠灌服生理盐水或相应剂量的GABA,14 d后采用高架十字迷宫实验(EPM)检测大鼠行为,然后采集血样,测定大鼠血浆甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平,并计算非高密度脂蛋白胆固醇(non-HDL-C)含量、TG/HDL-C、LDL-C/HDL-C比值。结果:应激后对照组体重和食物利用率极显著高于其余各组(P0.01);模型组进入开放臂的时间百分比极显著低于对照组和GABA 2 mg/kg组(P0.01),进入开放臂的次数百分比则显著低于对照组(P0.05);各组大鼠的入臂总次数差异不显著(P0.05)。血脂水平分析显示模型组血浆中TC含量显著高于GABA 2 mg/kg组(P0.05),non-HDL-C含量则显著高于对照组(P0.05),极显著高于GABA 2 mg/kg组(P0.01),模型组的LDL-C/HDL-C比值显著高于对照组和GABA 2 mg/kg组(P0.05)。结论 2 mg/kg GABA能明显增加EPM中进入开放臂的时间百分比,降低TC与non-HDL-C含量并下调LDL-C/HDL-C比值,有效缓解焦虑情绪并改善血脂代谢。
[Abstract]:Objective to investigate the effect of 纬-aminobutyric acid (GABA) on lipid metabolism in rats with emotional stress. Methods 40 male SD rats were randomly divided into control group, model group, GABA 0.5 (L), 1.0 (M) and 2.0 mg/kg (H) groups. Rats were given normal saline or corresponding dose of GABA,14 d before stress, and then the behavior of rats was detected by elevated maze test (EPM), and then the blood samples were collected to determine the total cholesterol (TC), of plasma triglyceride (TG), in rats. The levels of low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were measured, and the content of non-high density lipoprotein cholesterol (non-HDL-C) and the ratio of TG/HDL-C,LDL-C/HDL-C to cholesterol were calculated. Results: the body weight and food utilization rate in the control group after stress were significantly higher than those in the other groups (P0.01). The percentage of entering the open arm in the model group was significantly lower than that in the control group and GABA 2 mg/kg group (P0.01), and the percentage of entering the open arm in the model group was significantly lower than that in the control group (P0.05). There was no significant difference in the total frequency of entering arm among the groups (P0.05). Blood lipid level analysis showed that the plasma TC content in the model group was significantly higher than that in the GABA-2 mg/kg group (P0.05), while the non-HDL-C content in the model group was significantly higher than that in the control group (P0.05), very significantly higher than that in the GABA-2 mg/kg group (P0.01). The ratio of LDL-C/HDL-C in model group was significantly higher than that in control group and GABA-2 mg/kg group (P0.05). Conclusion 2 mg/kg GABA can significantly increase the percentage of time of entering open arm in EPM, decrease the content of TC and non-HDL-C and down-regulate the ratio of LDL-C/HDL-C, effectively relieve anxiety and improve the metabolism of blood lipid.
【作者单位】：华南农业大学食品学院广东省食品质量安全重点实验室;
【基金】：国家荔枝龙眼产业技术体系(No.CARS-33)
【分类号】：R363

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