反义锁核酸在转基因小鼠体内阻断乙肝病毒C基因表达

发布时间：2019-05-07 04:19

【摘要】：目的探讨针对乙肝病毒C基因反义锁核酸在转基因小鼠体内阻断病毒基因表达的效果。方法各实验组经尾静脉给药后,采用real-time PCR、时间分辨免疫荧光技术、免疫组织化学法等技术分别检测血清HBV DNA、HBeAg含量及肝细胞内HBcAg的表达情况。结果注射锁核酸后,对乙肝病毒核酸的复制和乙肝病毒e抗原的合成均有较强的抑制作用,注射后1、3和5 d,HBV DNA的平均抑制率分别19.24%、39.20%和44.47%;HBeAg的平均抑制分别为30.71%、51.14%和63.46%;小鼠肝细胞的HBcAg阳性细胞数也较对照组明显减少。结论针对乙肝病毒C基因的反义锁核酸在转基因小鼠体内能有效抑制病毒基因的复制和表达,提示C基因可作核酸药物治疗的有效靶位。
[Abstract]:Objective to investigate the effect of blocking the expression of HBV C gene in transgenic mice by antisense locking nucleic acid (ASODN) of hepatitis B virus C gene. Methods the levels of serum HBV DNA,HBeAg and the expression of HBcAg in hepatocytes were detected by time-resolved immunofluorescence (real-time PCR,) and immunohistochemistry (immunohistochemistry) after administration through caudal vein in each experimental group. Results the inhibition rate of HBV DNA was 19.24%, 39.20% and 44.47% respectively on the 1st, 3rd and 5th day after injection, and the inhibition rate of HBV DNA was 19.24%, 39.20% and 44.47%, respectively, and the inhibition rate of HBV DNA was 19.24%, 39.20% and 44.47% respectively at the 1st, 3rd and 5th day after injection. The average inhibition of HBeAg was 30.71%, 51.14% and 63.46%, respectively, and the number of HBcAg positive cells in mouse hepatocytes was also significantly decreased compared with the control group. Conclusion antisense locking nucleic acid targeting hepatitis B virus C gene can effectively inhibit the replication and expression of HBV gene in transgenic mice, suggesting that C gene can be used as an effective target for nucleic acid drug therapy.
【作者单位】：右江民族医学院附属医院医学检验中心;暨南大学第一附属医院医学检验中心;
【基金】：广西壮族自治区教育厅资助项目(200911MS187)
【分类号】：R373

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