小鼠肝癌休眠模型的建立
发布时间：2019-05-12 19:11
【摘要】：目的:建立小鼠肝癌休眠模型并验证休眠细胞的存在。方法:30只昆明种小鼠均右侧腋下接种1×106个H22腹水型肝癌细胞,常规喂养2周,第15天选择表皮肿瘤直径0.5cm的小鼠,进行姑息性手术,将肉眼可见肿瘤组织全部切除。手术后的小鼠再常规喂养8周,如接种处未见肿瘤组织生长,即认为肝癌小鼠休眠模型建立成功(按照平均寿命折算,小鼠生存期8周,即相当于人类寿命5年)。其后模型小鼠分为复发对照组和复发实验组,复发对照组小鼠常规喂养;复发实验组模型小鼠给予连续外伤刺激。6周后处死全部模型小鼠,接种部位取材,组织固定和HE染色观察肿瘤再生成情况确定休眠细胞的存在。结果:肝癌细胞接种于小鼠并行手术后,有20%小鼠成瘤,80%处于休眠状态。复发实验组给予连续外伤刺激6周后,100%小鼠成瘤,而复发对照组只有8.3%,两组差异有统计学意义,P=0.000。结论:本方法成功建立了小鼠肝癌休眠模型,并用外伤刺激引起肝癌休眠细胞增殖,验证了休眠肿瘤细胞在体内的存在。
[Abstract]:Objective: to establish a dormant model of liver cancer in mice and verify the existence of dormant cells. Methods: 30 Kunming mice were inoculated with 1 脳 106 H22 aspartate liver cancer cells under the right armpit and fed for 2 weeks. On the 15th day, the mice with 0.5cm in diameter of epidermis were selected for palliative operation and all the tumor tissues were resected with naked eye. If no tumor tissue growth was found in the inoculated mice, the dormancy model of liver cancer mice was established successfully (according to the average life expectancy, the survival time of mice was 8 weeks, that is, equivalent to 5 years of human life span). The model mice were divided into recurrent control group and recurrent experimental group, and the recurrent control group mice were fed with routine feeding. The model mice in the recurrent experimental group were stimulated by continuous trauma. After 6 weeks, all the model mice were killed and taken from the inoculated site. Tissue fixation and HE staining were used to observe the regeneration of the tumor to determine the existence of dormant cells. Results: after the liver cancer cells were inoculated in mice and operated, 20% of the mice formed tumors and 80% of them were dormant. After 6 weeks of continuous trauma stimulation, 100% of the mice in the recurrent experimental group formed tumors, while only 8.3% in the recurrent control group. There was significant difference between the two groups (P 鈮，
本文编号：2475629
[Abstract]:Objective: to establish a dormant model of liver cancer in mice and verify the existence of dormant cells. Methods: 30 Kunming mice were inoculated with 1 脳 106 H22 aspartate liver cancer cells under the right armpit and fed for 2 weeks. On the 15th day, the mice with 0.5cm in diameter of epidermis were selected for palliative operation and all the tumor tissues were resected with naked eye. If no tumor tissue growth was found in the inoculated mice, the dormancy model of liver cancer mice was established successfully (according to the average life expectancy, the survival time of mice was 8 weeks, that is, equivalent to 5 years of human life span). The model mice were divided into recurrent control group and recurrent experimental group, and the recurrent control group mice were fed with routine feeding. The model mice in the recurrent experimental group were stimulated by continuous trauma. After 6 weeks, all the model mice were killed and taken from the inoculated site. Tissue fixation and HE staining were used to observe the regeneration of the tumor to determine the existence of dormant cells. Results: after the liver cancer cells were inoculated in mice and operated, 20% of the mice formed tumors and 80% of them were dormant. After 6 weeks of continuous trauma stimulation, 100% of the mice in the recurrent experimental group formed tumors, while only 8.3% in the recurrent control group. There was significant difference between the two groups (P 鈮，
本文编号：2475629
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