胃癌腹腔种植转移模型小肠电生理变化及哇巴因干预效应

发布时间：2019-07-01 15:15

【摘要】：目的模拟胃癌腹腔种植转移模型,探讨胃肠恶性肿瘤晚期常常出现肠蠕动减慢,似肠梗阻症状,与小肠电生理的变化有无关系,应用哇巴因(ouabain)能否改善这种变化。方法 41只裸鼠分为4组,空白组5只,培养液组4只,SGC-7901胃癌细胞系建立模型32只(模型组8只,哇巴因处理24只作为用药组)。应用RM6240 B/C多道生物信号采集与处理系统进行数据采集。结果①模型鼠的电生理显示,其波幅和频率比正常裸鼠的低,且差异有统计学意义(P0.05)。②应用哇巴因进行干预后,模型鼠的波幅有明显升高(P0.01),频率有改变,但无统计学意义。③应用肿瘤细胞培养液的裸鼠电生理与正常比较显示,波幅明显升高(P0.01),频率无统计学意义。结论肿瘤在腹腔的种植转移能够降低小肠的电活动,应用哇巴因可以改善小肠的放电强度。
[Abstract]:Objective to simulate the model of abdominal implantation and metastasis of gastric cancer, and to investigate whether the symptoms of intestinal peristalsis and intestinal obstruction often occur in the late stage of gastrointestinal malignant tumors, which are related to the changes of small intestine electrophysiology. Whether ouabain (ouabain) can improve this change. Methods 41 nude mice were divided into 4 groups: blank group (n = 5), culture solution group (n = 4) and SGC-7901 gastric cancer cell line (n = 32) (model group (n = 8) and ouabain treatment (n = 24). RM6240 B / C multi-channel biological signal acquisition and processing system is used for data acquisition. Results 1 the electrophysiological results of the model mice showed that the amplitude and frequency of the model mice were lower than those of the normal nude mice, and the difference was statistically significant (P 0.05). 2 after the intervention with ouabain, the amplitude of the model mice increased significantly (P 0.01), and the frequency changed, but there was no statistical significance. 3 the electrophysiology of nude mice treated with tumor cell culture medium showed that the amplitude of the nude mice was significantly higher than that of the normal nude mice (P 0.01), and the frequency was not statistically significant. Conclusion implantation and metastasis of tumor in abdominal cavity can reduce the electrical activity of small intestine, and ouabain can improve the discharge intensity of small intestine.
【作者单位】：哈尔滨医科大学病理学教研室;
【基金】：黑龙江省教育厅重点项目(12521z017)
【分类号】：R-332

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