海马TLR4及TNF-α参与CCI大鼠神经痛的初步研究
发布时间：2021-06-05 04:20
目的:1.阐明海马TLR4（Toll like receptor 4,Toll样受体4）及TNF-α（Tumor Necrosis Factor-alpha,肿瘤坏死因子-α）表达是否参与CCI（chronic constriction injury of sciatic nerve,坐骨神经慢性缩窄性损伤）大鼠神经痛。2.阐明双侧海马注射米诺环素或miRNA-181c激动剂能否通过下调海马TLR4及TNF-α表达缓解CCI大鼠神经痛。方法:选用56周龄SD雄性大鼠,均行海马CA1区置管。1.SD大鼠,随机分成3组（n=8）:（1）假手术组（Sham组）、（2）CCI+溶剂对照组（CCI+0.01 mol/L PBS）、（3）CCI+米诺环素组（CCI+2μg/μL米诺环素）。CCI+米诺环素组双侧海马CA1区注射米诺环素（2μg/μL,2次/日,持续7 d）。假手术组及CCI+溶剂对照组双侧海马CA1区注射等体积的0.01M PBS（持续7 d）。于术前1 d,术后第1、3、5、7 d测定给药1 h后的MWT（mechanical withdraw thresh...
【文章来源】：遵义医科大学贵州省
【文章页数】：60 页
【学位级别】：硕士
【部分图文】：
背侧海马CA1微量注射定位图
后 1、3、5、7 d MWT 明显延长，差异具有统计学意义（P<0.05），表现出良好的镇痛效应。图2 海马内注射米诺环素对CCI大鼠MWT的影响Fig. 2 The changes of MWT on the ipsilateral side after nerve injury in every group at different days(n = 8).aP <0.01, compared with sham group;bP <0.05, compared with CCI group.3.4 CA1 区注射米诺环素对 CCI 大鼠海马 Iba-1、TLR2、TLR4、IL-1β、TNF-α和 iNOS mRNA 表达的影响Fig. 3为RTFQ-PCR实验中的扩增曲线与熔解曲线。如图Fig. 4所示，与假手术组相比，CCI术后7d，CCI组大鼠海马Iba-1、TLR2、TLR4、IL-1β、TNF-α和iNOS mRNA表达明显上调，差异具有统计学意义（P<0.01）。海马CA1区注射米诺环素几乎完全抑制坐骨神经损伤诱发的海马Iba-1、TLR2、TLR4、TNF-α mRNA表达上调（与CCI组相比，P<0.01；与假手术组相比无统计学意义，P>0.05），仅部分阻断CCI大鼠海马IL-1β和iNOS mRNA表达上调（与CCI组相比
23图3 RTFQ-PCR实验中的扩增曲线与熔解曲线ion kinetics curves and Melting curves in RTFQ-PCR experiments:nd Melting curves for β-actin mRNA. A1：Amplication kinetics celting curves for β-actin mRNA. B: Amplication kinetics curve and. B1: Amplication kinetics curvefor Iba-1 mRNA. B2: Melting plication kinetics curve and Melting curves for TLR2 mRNA. C1r TLR2 mRNA. C2: Melting curves for TLR2 mRNA. D: Amplicatiorves for TLR4 mRNA. D1: Amplication kinetics curve TLR4 mRN4 mRNA. E: Amplication kinetics curve and Melting curves for ILetics curve for IL-1β mRNA. E2: Melting curves for IL-1β mRNAnd Melting curves for TNF-α mRNA. F1: Amplication kinetics clting curves for TNF-α mRNA. G: Amplication kinetics curve and MG1: Amplication kinetics curve for iNOS mRNA. G2: Melting c
本文编号：3211422
【文章来源】：遵义医科大学贵州省
【文章页数】：60 页
【学位级别】：硕士
【部分图文】：
背侧海马CA1微量注射定位图
后 1、3、5、7 d MWT 明显延长，差异具有统计学意义（P<0.05），表现出良好的镇痛效应。图2 海马内注射米诺环素对CCI大鼠MWT的影响Fig. 2 The changes of MWT on the ipsilateral side after nerve injury in every group at different days(n = 8).aP <0.01, compared with sham group;bP <0.05, compared with CCI group.3.4 CA1 区注射米诺环素对 CCI 大鼠海马 Iba-1、TLR2、TLR4、IL-1β、TNF-α和 iNOS mRNA 表达的影响Fig. 3为RTFQ-PCR实验中的扩增曲线与熔解曲线。如图Fig. 4所示，与假手术组相比，CCI术后7d，CCI组大鼠海马Iba-1、TLR2、TLR4、IL-1β、TNF-α和iNOS mRNA表达明显上调，差异具有统计学意义（P<0.01）。海马CA1区注射米诺环素几乎完全抑制坐骨神经损伤诱发的海马Iba-1、TLR2、TLR4、TNF-α mRNA表达上调（与CCI组相比，P<0.01；与假手术组相比无统计学意义，P>0.05），仅部分阻断CCI大鼠海马IL-1β和iNOS mRNA表达上调（与CCI组相比
23图3 RTFQ-PCR实验中的扩增曲线与熔解曲线ion kinetics curves and Melting curves in RTFQ-PCR experiments:nd Melting curves for β-actin mRNA. A1：Amplication kinetics celting curves for β-actin mRNA. B: Amplication kinetics curve and. B1: Amplication kinetics curvefor Iba-1 mRNA. B2: Melting plication kinetics curve and Melting curves for TLR2 mRNA. C1r TLR2 mRNA. C2: Melting curves for TLR2 mRNA. D: Amplicatiorves for TLR4 mRNA. D1: Amplication kinetics curve TLR4 mRN4 mRNA. E: Amplication kinetics curve and Melting curves for ILetics curve for IL-1β mRNA. E2: Melting curves for IL-1β mRNAnd Melting curves for TNF-α mRNA. F1: Amplication kinetics clting curves for TNF-α mRNA. G: Amplication kinetics curve and MG1: Amplication kinetics curve for iNOS mRNA. G2: Melting c
本文编号：3211422
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