组蛋白赖氨酸甲基转移酶2D调控H9c2细胞中HIF-1α/VEGF通路（英文）
发布时间：2022-07-23 14:52
组蛋白赖氨酸甲基转移酶2D （histone-lysine N-methyltransferase 2D, KMT2D）作为主要的组蛋白3第4位赖氨酸（H3K4）甲基转移酶,在调控胚胎发育、组织分化、代谢和肿瘤抑制方面发挥重要作用。在小鼠体内,敲除Kmt2d会导致严重的心脏发育缺陷最终造成胚胎期死亡。低氧诱导因子-1α（hypoxia-inducible factor 1α, HIF-1α）作为调节细胞应对低氧的关键转录因子,能够调控多种下游基因转录。有相关研究揭示,表观遗传调控者能够调节HIF-1α的稳定性和活性。同样,作为表观遗传调控者的组蛋白甲基转移酶KMT2D是否参与低氧条件下HIF-1α对下游基因的调控,目前仍未知。在本研究中,观察在Kmt2d正常或缺乏的情况下,心肌细胞H9c2对低氧环境的应答反应。结果显示,与常氧条件相比,低氧状态下HIF-1α、组蛋白乙酰化酶P300、KMT2D及其介导的H3K4一甲基化（H3K4 mono-methylation, H3K4me1）的蛋白质水平增加（P<0.05）;HIF-1α下游基因血管内皮生长因子（vascular endot...
【文章页数】：7 页
【文章目录】：
1 Materials and Methods
1.1 Cells culture and treatment
1.2 Nuclear extraction
1.3 Western blot
1.4 qRT-PCR
1.5 ChIP-qPCR
1.6 Gene silencing of Kmt2d
1.7 Statistical analysis
2 Results
2.1 Increased KMT2D, HIF-1α, P300, and H3K4me1 protein expression in H9c2 cells under hypoxic condition
2.2 Enhanced mRNA expression of Vegf and enrichment of H3K4me1 and H3K27ac on Vegf promoter in H9c2 cells under hypoxic condition
2.3 Knockdown of Kmt2d reduced the accumulation of HIF-1α and the expression of Vegf under hypoxic condition
3 Discussion
本文编号：3665341
【文章页数】：7 页
【文章目录】：
1 Materials and Methods
1.1 Cells culture and treatment
1.2 Nuclear extraction
1.3 Western blot
1.4 qRT-PCR
1.5 ChIP-qPCR
1.6 Gene silencing of Kmt2d
1.7 Statistical analysis
2 Results
2.1 Increased KMT2D, HIF-1α, P300, and H3K4me1 protein expression in H9c2 cells under hypoxic condition
2.2 Enhanced mRNA expression of Vegf and enrichment of H3K4me1 and H3K27ac on Vegf promoter in H9c2 cells under hypoxic condition
2.3 Knockdown of Kmt2d reduced the accumulation of HIF-1α and the expression of Vegf under hypoxic condition
3 Discussion
本文编号：3665341
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