成熟中性粒细胞中淀粉样变纤维成分的检测

发布时间：2018-01-08 09:28

本文关键词：成熟中性粒细胞中淀粉样变纤维成分的检测　出处：《实用医学杂志》2016年14期 　论文类型：期刊论文

【摘要】：目的:先前报道红细胞中存在淀粉样纤维,本文进一步探讨成熟中性粒细胞是否存在淀粉样变纤维,为解析阿尔茨海默病(AD)淀粉样变形成分子机制提供科学依据。方法:收集AD患者以及对照人群各30例,利用希森美康X5-500i血液分析仪测定血液白细胞、红细胞、血红蛋白水平以及中性粒细胞绝对值等4项指标,采集静脉EDTA-K_2抗凝血分离中性粒细胞,分别测定中性粒细胞与淀粉样变特异探针之间亲和性,血浆部分用硫磺素T(ThT)检测淀粉样变荧光强度。结果:亲和试验结果显示淀粉样变荧光探针(ThT)能够与组织淀粉样变纤维特异性结合,中性粒细胞中淀粉样变纤维染色也呈现阳性反应。AD组与健康对照组间血细胞白细胞、红细胞、血红蛋白水平以及中性粒细胞绝对值比较差异无统计学意义(P0.05),但是血浆淀粉样变荧光强度显著高于对照组(P0.05)。结论:报道成熟中性粒细胞发现淀粉样变纤维,且AD患者血浆淀粉样变纤维水平显著增高。
[Abstract]:Objective: previous reports exist of amyloid fibrils in red blood cells, this paper further explores the existence of mature neutrophils to amyloid fibrils, for the analysis of Alzheimer's disease (AD) amyloid formation molecular mechanism to provide scientific basis. Methods: collect AD patients and control group 30 cases, determination of white blood cell, using Sysmex X5-500i of RBC, hemoglobin level and 4 indexes of neutrophils, collecting venous EDTA-K_2 anticoagulant isolated neutrophils, neutrophils were determined with amyloid specific probe affinity between plasma respectively, with Thioflavin T (ThT) to detect amyloid fluorescence intensity. Results: affinity test the results showed that amyloid fluorescence probe (ThT) can be combined with tissue amyloid fibers, neutrophil amyloid fiber staining also showed positive reaction in.AD group With the healthy control group between blood cells and white blood cells, red blood cells, hemoglobin level and absolute neutrophil count had no statistically significant difference (P0.05), but serum amyloid fluorescence intensity was significantly higher than the control group (P0.05). Conclusion: reports of mature neutrophils are amyloid fibrils, and plasma AD of patients with starch like fiber level increased significantly.

【作者单位】：天津医科大学医学检验学院;天津医科大学总医院神经内科;
【基金】：天津市自然基金面上项目(编号:15JCYBJC26800)
【分类号】：R749.16
【正文快照】： 随着人口老龄化的日益明显,阿尔茨海默病(Alzheimer′s disease,AD)成为危害老年人健康的危害因素之一,预测到2030年AD患者将达到660万[1]。AD是一种进行性的神经退行性疾病,临床表现为认知和记忆衰退,研究证实[2-3]脑组织和血管中有淀粉样变纤维成分,并且淀粉样变成分多为β

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