贵州省精神分裂症患者弓形虫感染状况调查及其基因型鉴定

发布时间：2018-01-14 06:31

本文关键词：贵州省精神分裂症患者弓形虫感染状况调查及其基因型鉴定　出处：《贵阳医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的用免疫学和分子生物学方法调查贵州省精神分裂症患者弓形虫的感染状况，并用多重巢式-聚合酶链反应-限制性片段长度多态性(Mn-PCR-RFLP)方法进行弓形虫基因分型，探讨精神分裂症与弓形虫感染的关系以及贵州省弓形虫感染的分子流行病学特点。方法采集112份精神分裂症患者（疾病组）和101份正常人群（对照组）外周血标本。采用ELISA法检测外周血血浆中弓形虫循环抗原(CAg)及抗弓形虫特异性IgM、IgG抗体。应用PCR技术扩增外周血弓形虫的特异性基因片段(529bp)，检测弓形虫核酸。对于弓形虫核酸检测阳性的标本用Mn-PCR-RFLP法分析弓形虫c22-8， c29-2，， L358，PK1，SAG2(5`-SAG2，3`-SAG2，alt.SAG2)，BTUB，GRA6，SAG3，Apico，SAG1共10个不同基因位点，鉴定弓形虫的基因型，分析贵州省精神分裂症患者弓形虫感染的分子流行病学特点。结果112份精神分裂症患者标本中检测到弓形虫特异性IgG抗体阳性18例， CAg阳性1例，弓形虫核酸阳性6例，101份正常人群标本中检测到弓形虫特异性IgG抗体阳性7例， CAg阳性1例。精神分裂症患者弓形虫感染率为22.3%，与对照组（7.9%）比较，差异具有统计学意义(P=0.0042)。测得的OR值为3.391，95%CI=1.420-8.097。Mn-PCR-RFLP鉴定6份标本中弓形虫的基因型一致,属于非典型虫株ToxoDB#9。结论精神分裂患者弓形虫感染率高于正常人群，提示精神分裂症的发生可能与弓形虫感染有关。贵州省精神分裂症患者感染的弓形虫属于不典型基因型Chinese1。
[Abstract]:Objective to investigate the infection of Toxoplasma gondii in schizophrenic patients in Guizhou Province by immunological and molecular biological methods. The genotyping of Toxoplasma gondii was carried out by multiplex nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP). To explore the relationship between schizophrenia and Toxoplasma gondii infection and the molecular epidemiological characteristics of toxoplasmosis infection in Guizhou Province. Methods 112 schizophrenic patients (disease group) and 101 normal persons (control group) were collected. The circulating antigen of Toxoplasma gondii (Toxoplasma gondii) and anti-Toxoplasma gondii (Toxoplasma gondii) specific IgM were detected by ELISA method. IgG antibody. The specific gene fragment of Toxoplasma gondii was amplified by PCR. Toxoplasma gondii nucleic acid was detected. Toxoplasma gondii c22-8, c29-2, L358PK1 were analyzed by Mn-PCR-RFLP method. SAG2(5 '-SAG2O3`-SAG2Alt.SAG2ANG2, BTUBMAGRA6, SAG3, Apicotrol SAG1 have 10 different gene loci. Identification of Toxoplasma gondii genotypes. The molecular epidemiologic characteristics of Toxoplasma gondii infection in schizophrenic patients in Guizhou Province were analyzed. Results 18 cases of Toxoplasma gondii specific IgG antibody were detected in 112 samples of schizophrenic patients. CAg was positive in 1 case, Toxoplasma nucleic acid positive in 6 cases and Toxoplasma gondii specific IgG antibody in 7 cases. The infection rate of Toxoplasma gondii in schizophrenic patients was 22. 3%, which was compared with that in control group (7. 9%). The difference was statistically significant. The OR was 3.391. The genotypes of Toxoplasma gondii were identical in 6 samples identified by 95CII 1.420-8.097.Mn-PCR-RFLP. Conclusion Toxoplasma gondii infection rate in schizophrenic patients is higher than that in normal controls. The results suggest that the occurrence of schizophrenia may be related to Toxoplasma gondii infection. Toxoplasma gondii infected by schizophrenic patients in Guizhou Province belongs to the atypical genotype Chinese1.
【学位授予单位】：贵阳医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R749.3;R531.8

【参考文献】