褪黑素对阿尔茨海默病大鼠学习记忆障碍的影响及作用机制

发布时间：2018-02-12 13:28

本文关键词： 褪黑素东莨菪碱学习记忆皮层长时程增强　出处：《中国老年学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨褪黑素(MT)对阿尔茨海默病(AD)大鼠学习记忆障碍的影响及作用机制。方法 SD大鼠随机分为3组:正常对照组、东莨菪碱模型组和MT组。正常对照组和模型组每天腹腔注射生理盐水2 ml;MT组每天腹腔注射MT溶剂(2 mg/kg)2 ml,连续给药7 d,于第8天进行Morris水迷宫测试,测试6 d,每天测试前30 min各组(正常对照组腹腔注射等剂量的生理盐水)腹腔注射东莨菪碱1 mg/kg。于第15天开始进行电生理记录。结果①水迷宫测试6 d后,模型组逃避潜伏期的时间(61.86±8.84)s,与正常对照组(11.13±6.08)s相比,缩短趋势明显较慢(P0.01);MT组逃避潜伏期为(16.250±10.926)s,与正常对照组相比无显著差异(P0.05)。水迷宫测试的第6天,模型组大鼠的穿环次数为(1.91±0.87)次,较正常对照组(3.34±0.69)次明显减少(P0.01);MT组大鼠的穿环次数为(3.15±0.72)次,与正常对照组相比无显著差异(P0.05)。②给予100 Hz 1 s刺激后,正常对照组诱发反应的幅度为基准值的(156.36±9.69)%,MT组为(142.25±11.03)%,两组与刺激前相比均有显著差异(P0.01),模型组的幅度为基准值的(108.56±12.41)%,与刺激前相比无显著差异(P0.05);给予100Hz 1 s刺激后,正常对照组诱发反应的斜率为基准值的(137.31±13.74)%,MT组为(130.59±13.65)%,两组与刺激前相比均有显著差异(P0.01),模型组的斜率为基准值的(101.36±11.72)%,与刺激前相比无显著性差异(P0.05)。结论 MT对东莨菪碱所致的大鼠学习记忆障碍有明显的改善作用,并且可以阻断东莨菪碱对皮层长时程增强的抑制。
[Abstract]:Objective to investigate the effect and mechanism of melatonin (MT) on learning and memory impairment in rats with Alzheimer's disease (AD). Methods SD rats were randomly divided into three groups: normal control group. Scopolamine model group and MT group, normal control group and model group were injected intraperitoneally with normal saline 2 ml / day and MT group intraperitoneally injected MT solvent 2 mg/kg)2 / ml daily. After 7 days of continuous administration, Morris water maze test was performed on the 8th day. The rats were given scopolamine 1 mg / kg intraperitoneally 30 min before each day (normal control group was injected with saline of the same dose) for 6 days. The electrophysiological records were recorded on the 15th day. Results 1 after 6 days of water maze test, the rats were injected with scopolamine 1 mg / kg. The escape latency of the model group was 61.86 卤8.84 s, which was significantly slower than that of the normal control group (11.13 卤6.08 s). In the MT group, the escape latency was 16.250 卤10.926 seconds, and there was no significant difference between the model group and the normal control group (P 0.05). On the 6th day of water maze test, the number of piercing times in the model group was 1.91 卤0.87). Compared with the normal control group, 3.34 卤0.69 times decreased significantly the number of piercing times of the rats in the P0.01MT-treated group was 3.15 卤0.72 times, and there was no significant difference between the control group and the normal control group after 100 Hz 1 s stimulation. The amplitude of evoked response in the normal control group was 156.36 卤9.69g / kg, which was 142.25 卤11.03g in MT group. There was significant difference between the two groups compared with before stimulation (P 0.01). The amplitude of model group was 108.56 卤12.41% of the baseline value, but there was no significant difference compared with that before stimulation (P 0.05), and after 100 Hz 1 s stimulation, there was no significant difference between the two groups. The slope of the induced response in the normal control group was 137.31 卤13.74 and that in the MT group was 130.59 卤13.650.The slope of the model group was 101.36 卤11.72, and the slope of the model group was 101.36 卤11.72, and there was no significant difference between the two groups in scopolamine. Conclusion MT has no significant difference on scopolamine. In rats with learning and memory impairment, And it can block the inhibition of scopolamine on long-term cortical enhancement.
【作者单位】：南华大学医学院基础医学部;漯河医学高等专科学校基础医学部;阳谷县第一人民医院康复科;
【基金】：河南省科技厅基础与前沿技术研究(152300410014) 漯河医学等专科学校2015年度基础科学研究项目(2015-S-LMC06)
【分类号】：R749.16

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