L-茶氨酸改善慢性应激大鼠抑郁行为作用研究

发布时间：2018-02-13 08:58

本文关键词： L-茶氨酸抑郁慢性不可预见性温和应激改善作用　出处：《茶叶科学》2014年04期 　论文类型：期刊论文

【摘要】：研究L-茶氨酸对慢性不可预见性温和应激模型大鼠的抗抑郁作用及其机制。建立慢性不可预见性温和应激(CUMS)抑郁大鼠模型,通过糖水偏好实验,旷场实验,明暗箱实验评估L-茶氨酸的抗抑郁作用效果;为了阐释其相关机理,本研究通过酶联免疫法测定了各组大鼠海马和皮层组织中5-羟色胺(5-HT)、去甲肾上腺素(NA)、促肾上腺皮质激素(ACTH)和皮质酮(CORT)的含量,同时也检测了大鼠血清和海马组织中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性。结果表明,与空白对照组相比,模型组大鼠糖水偏好度、横穿格数和直立次数、进入明箱中的大鼠数量以及停留的时间极显著降低,显示大鼠抑郁模型构建成功;而与模型组相比,灌胃低高两个剂量L-茶氨酸的大鼠抑郁症状有不同程度改善,尤其高剂量都达到显著水平。同时,L-茶氨酸能显著提升大鼠海马和皮层中5-HT、NA的含量,降低ACTH,CORT的含量,上调大鼠血清和皮层中SOD和GSH-Px的活性,进一步表明了其抗抑郁的功效,其机制可能主要与促进单胺类递质分泌有关。
[Abstract]:To study the antidepressant effect of L-theanine on chronic unpredictable mild stress rats and its mechanism. To evaluate the antidepressant effect of L-theanine, and to elucidate its mechanism, The contents of 5-hydroxytryptamine (5-HT), norepinephrine (NAA), adrenocorticotropic hormone (ACTH) and corticosterone (Cort) in hippocampal and cortical tissues of rats in each group were determined by enzyme-linked immunosorbent assay (Elisa). The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum and hippocampus of rats were also measured. The number of rats entering the tank and the length of stay were significantly decreased, indicating that the depression model was successfully constructed, while the depression symptoms of rats with low dose of L-theanine were improved in different degrees compared with the model group. At the same time, L-theanine could significantly increase the content of 5-HTNNA in hippocampus and cortex of rats, decrease the content of ACTHCORT, and up-regulate the activities of SOD and GSH-Px in serum and cortex of rats, which further indicated its antidepressant effect. The mechanism may be related to the secretion of monoamine transmitters.
【作者单位】：国家植物功能成分利用工程技术研究中心;湖南农业大学茶学教育部重点实验室;
【基金】：湖南省科技计划项目(2014FJ3131) 国家自然科学基金项目(31100502) 国家茶叶产业技术体系项目(CARS-23-10B) 湖南省教育厅优秀青年项目(12B064)
【分类号】：R749.4

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