自噬对血管性痴呆大鼠海马CA1区突触素及突触后膜致密物质95表达的影响

发布时间：2018-02-21 20:51

本文关键词： 血管性痴呆自噬突触素突触后膜致密物质突触可塑性　出处：《第二军医大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨自噬对血管性痴呆大鼠海马CA1区突触素(synaptophysin,Syn)及突触后膜致密物质95(postsynaptic density material 95,PSD-95)表达的影响。方法健康雄性SD大鼠96只,随机分为假手术组(Sham组)、血管性痴呆模型组(VD组)、自噬抑制剂3-甲基腺嘌呤预处理组(3-MA组)和自噬激动剂雷帕霉素预处理组(Rap组)。每组又分为模型制备成功后1、2、4、8周4个亚组,每个亚组6只大鼠。应用改良的Pulsinelli四血管阻断法制备血管性痴呆模型,采用蛋白质印迹法检测大鼠海马CA1区微管相关蛋白1轻链3(LC3)、Syn、PSD-95蛋白表达。结果(1)与Sham组比较,VD组各时间点LC3-Ⅱ/LC3-Ⅰ比值增加,Syn、PSD-95蛋白表达均减少,差异有统计学意义(P均0.01);与VD组比较,3-MA组各时间点LC3-Ⅱ/LC3-Ⅰ比值减少,Syn、PSD-95蛋白表达增加(P0.05或P0.01),Rap组各时间点LC3-Ⅱ/LC3-Ⅰ比值增加,Syn、PSD-95蛋白表达降低(P均0.01)。(2)自噬相关蛋白LC3-Ⅱ/LC3-Ⅰ比值与Syn、PSD-95蛋白表达呈负相关(P均0.01)。结论自噬对血管性痴呆大鼠海马CA1区突触可塑性相关蛋白Syn、PSD-95表达具有抑制作用,抑制自噬有利于血管性痴呆大鼠海马CA1区神经突触重塑。
[Abstract]:Objective to investigate autophagy in hippocampal CA1 region of vascular dementia rats synaptophysin (synaptophysin, Syn) and postsynaptic density 95 (postsynaptic density material 95, PSD-95) was studied. Methods 96 healthy male SD rats were randomly divided into sham operation group (group Sham), vascular dementia model group (VD group), autophagy inhibitor 3- methyladenine pretreatment group (group 3-MA) and autophagy agonist rapamycin pretreatment group (Rap group). Each group was divided into 1,2,4,8 weeks after the model was successfully established 4 subgroups, 6 rats in each sub group. The application of the modified Pulsinelli four vessel occlusion vascular dementia rat model by using Western blotting of microtubule associated protein in rat hippocampal CA1 region was detected in 1 light chain 3 (LC3), Syn, expression of PSD-95 protein. Results (1) compared with Sham group, VD group at different time points of LC3- II /LC3- II ratio increases, Syn, PSD-95 protein expression was decreased, there was statistically significant difference The meaning of (P 0.01); compared with VD group, 3-MA group at different time points of LC3- II /LC3- II ratio decreased, Syn increased the expression of PSD-95 (P0.05 or P0.01), Rap group at each time point LC3- II /LC3- II ratio increases, Syn, PSD-95 protein expression decreased (P < 0.01). (2). The related protein LC3- II /LC3- II ratio and Syn, PSD-95 protein expression was negatively correlated (P < 0.01). Conclusion autophagy on vascular dementia synaptic plasticity in rat hippocampal CA1 region associated protein Syn inhibits PSD-95 expression, inhibition of autophagy in favor of vascular dementia rat hippocampal CA1 synaptic remodeling.

【作者单位】：华北理工大学附属医院神经内一科;
【基金】：河北省高等学校科学技术研究重点项目(ZH2012046) 河北省重大医学科研课题(zd2013087)~~
【分类号】：R749.13

【相似文献】