丙酮酸盐对阿尔茨海默病大鼠学习记忆的影响

发布时间：2018-02-24 21:55

本文关键词： 丙酮酸盐阿尔茨海默病学习记忆　出处：《中国神经精神疾病杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的研究丙酮酸盐对阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠学习记忆的影响并探讨其与海马神经细胞死亡的关系。方法将36只wistar大鼠随机分为空白对照组、AD模型组和丙酮酸盐治疗组,根据实验要求给药处理6周,同时观察各组大鼠一般情况,Morris水迷宫实验检测大鼠学习记忆能力,苏木精-伊红(hematoxylin and eosin,HE)染色检测海马神经细胞死亡,利用2′,7′-dichlorodihydrofluorescein diacetate(H2DCF-DA)来检测海马CA1区内活性氧(reactive oxygen species,ROS)聚集情况。结果丙酮酸盐治疗组与AD模型组比较,一般情况均明显改善,逃避潜伏期均明显缩短(41.37±6.24,31.67±7.26,24.26±7.16,20.45±5.72,19.36±5.89,均P0.05);原平台象限活动时间明显增加(50.36±7.46 s,P0.05),穿越原平台次数明显增多(3.98±1.23次/2 min,P0.05),海马CA1区锥体神经细胞死亡显著减少(P0.05),海马神经细胞内ROS的水平也显著降低(P0.05)。结论丙酮酸盐可能通过降低海马组织内ROS的产生,减少海马神经细胞死亡,从而提高AD模型大鼠的学习记忆水平。
[Abstract]:Objective to study the effect of pyruvate on learning and memory in rats with Alzheimer's disease (AD) and to explore the relationship between pyruvate and hippocampal nerve cell death. Methods 36 wistar rats were randomly divided into control group and pyruvate treatment group. According to the experimental requirements, the rats were treated for 6 weeks, and their learning and memory abilities were measured by Morris water maze test and hematoxylin and eosin HEH staining to detect the death of hippocampal nerve cells. The aggregation of reactive oxygen speciess-ROSs in the hippocampal CA1 was detected by using 2-dichlorodihydrofluorescein diacetate H2DCF-DA.Results the general situation of pyruvate treatment group was significantly improved compared with that of AD model group. The latency of escape was significantly shortened by 41.37 卤6.24s31.67 卤7.26tl 24.26 卤7.162n 20.45 卤5.72n 19.36 卤5.89m, and the time of activity in the original platform quadrant was significantly increased by 50.36 卤7.46sm P0.05n, and the number of times of crossing the original platform was increased by 3.98 卤1.23 times per second minute P0.05. the death of pyramidal neurons in hippocampal CA1 region significantly decreased P0.05a, and the ROS of hippocampal neurons in the hippocampal neurons decreased significantly. Conclusion Pyruvate may decrease the production of ROS in hippocampal tissue. Decrease the death of hippocampal nerve cells and improve the learning and memory level of AD model rats.
【作者单位】：中国医科大学附属第一医院呼吸感染科;
【基金】：国家自然科学基金资助项目(编号:81200246) 高等学校博士学科点专项科研基金(编号:20122104120004) 辽宁省自然科学基金项目(编号:2013010025-401)
【分类号】：R749.16

【参考文献】