血栓心脉宁对β-淀粉样蛋白致大鼠老年性痴呆的治疗作用

发布时间：2018-03-01 19:44

本文关键词： 血栓心脉宁老年性痴呆 β-淀粉样蛋白大鼠 Wistar　出处：《吉林大学学报(医学版)》2014年05期 　论文类型：期刊论文

【摘要】：目的:观察血栓心脉宁对β-淀粉样蛋白(Aβ)致老年性痴呆(AD)大鼠行为学及大脑皮层和海马组织结构的影响,探讨血栓心脉宁对大鼠AD的治疗作用。方法:选取100只雄性Wistar大鼠,按体质量将大鼠随机分为假手术组、模型组、阳性药组(盐酸多奈哌齐,1.75mg·kg-1)和血栓心脉宁1.1、2.2g·kg-1剂量组。连续灌胃给药15d。大鼠海马定位注射Aβ建立大鼠AD模型。进行Morris水迷宫试验、避暗及病理学实验。结果:水迷宫试验,与模型组比较,血栓心脉宁1.1g·kg-1剂量组大鼠第2、4和5天到达平台的潜伏期和游程明显缩短(P0.05或P0.01);血栓心脉宁2.2g·kg-1剂量组大鼠第3~6天到达平台的潜伏期缩短(P0.05或P0.01),第3~5天到达平台的游程明显缩短(P0.05或P0.01);第7天血栓心脉宁各剂量组大鼠在90s内经过平台的次数、平台停留时间、平台区停留距离、有效区停留时间、有效区停留距离、平台停留时间/总时间、平台停留距离/总路程和有效区停留时间/总时间均明显增加(P0.05或P0.01)。避暗实验中,与模型组比较,血栓心脉宁各剂量组大鼠第2天的错误潜伏期及错误次数无显著差异(P0.05)。病理学观察,血栓心脉宁各剂量组大鼠与模型组比较其病理学表现无明显差异,大脑皮层神经细胞有变性、坏死;海马神经细胞数少,层次不清楚,可见变性、坏死细胞。结论:血栓心脉宁可改善AD大鼠的学习记忆功能,但对其病理学病变无明显改善作用。
[Abstract]:Objective: to observe the effect of Xuexinmaining on the behavior, cerebral cortex and hippocampal tissue structure of AD rats induced by 尾 -amyloid protein A 尾, and to explore the therapeutic effect of Xuexinmaining on AD in rats. Methods: 100 male Wistar rats were selected. Rats were randomly divided into sham-operation group, model group, positive drug group (Donepezil hydrochloride 1.75mg 路kg-1) and thromboxinine 1.1g 路kg-1 group according to body mass. The rats were injected with A 尾 in hippocampus for 15 days to establish AD model. Morris water maze test was performed. Dark avoidance and pathological experiments. Results: water maze test, compared with the model group, In Xuexinmaining 1.1g 路kg-1 group, the latency and travel time to the platform were significantly shortened on the 4th and 5th day, P0.05 or P0.01, respectively, while the incubation period of Xuexinmaining 2.2g 路kg-1 group was shortened by P05 or P0.01a on the 6th day, and reached the platform on the 35th day by the dose of Xuexinmaining (2.2g 路kg-1). On the 7th day, the rats of each dose group of Xuexin Maining passed through the platform within 90 s, the course was significantly shorter than that of the control group (P 0.05 or P 0.01). Platform stay time, platform area stay distance, effective area stay time, effective area stay distance, platform stay time / total time, The length of stay / total distance of platform and the residence time of effective area / total time were significantly increased by P0.05 or P0.01. in the dark avoidance experiment, compared with the model group, There was no significant difference in the error latency and the number of errors in the rats of each dose group of Xuexinmaining on the second day (P 0.05). There was no significant difference in the pathological manifestations of the rats in each dose group of Xuexinmaining compared with the model group, and the pathological changes of the rats in each dose group were similar to those in the model group. The number of hippocampal neurons is small, the level is unclear, the degeneration and necrosis cells can be seen. Conclusion: Xuexinmai can improve the learning and memory function of AD rats. However, there was no obvious improvement on pathological changes.
【作者单位】：吉林大学药学院药理学教研室;
【基金】：吉林省中医药管理局科研基金资助课题(2010-115)
【分类号】：R749.16

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