单次鼻腔给予胰岛素对异丙酚引起的tau蛋白过度磷酸化影响的实验研究

发布时间：2018-03-08 01:16

本文选题：胰岛素　切入点：异丙酚　出处：《华中科技大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：探讨单次鼻腔给予胰岛素对异丙酚引起的tau蛋白过度磷酸化的影响及机制。方法：SD大鼠，雄性,8周，给予以下处理：鼻腔给予胰岛素（3ml:300IU）或生理盐水42μl,半小时后腹腔注射异丙酚150mg/kg或等体积脂肪乳，一个小时后颈椎脱臼法处死，取双侧海马组织，通过Westernblot检测tau蛋白Thr205位点的磷酸化，GSK-3β、AKT、ERK、PP2A活性的变化。结果：异丙酚引起tau蛋白Thr205位点的过度磷酸化,这种变化与磷酸激酶GSK-3β、AKT、ERK的活性变化关系不大，而主要可能由于磷酸酶PP2A总量下降所致。单次鼻腔给予胰岛素并没有逆转异丙酚引起tau蛋白Thr205位点的过度磷酸化。结论：单次鼻腔给予胰岛素并不能对异丙酚引起的相关异常变化产生显著的保护作用，，采用胰岛素作为术后认知功能障碍的保护措施尚需进一步研究。
[Abstract]:Aim: to investigate the effect and mechanism of single nasal insulin administration on propofol induced hyperphosphorylation of tau protein. Methods male SD rats were given the following treatment for 8 weeks: nasal administration of insulin (3ml: 300 IUU) or normal saline (42 渭 l), intraperitoneal injection of propofol 150 mg / kg or fat emulsion of equal volume half an hour later, cervical spine dislocations were performed one hour later, and bilateral hippocampal tissues were taken. The activity of PP2A at Thr205 site of tau protein was detected by Westernblot. Results: propofol induced excessive phosphorylation of Thr205 site of tau protein. This change was not related to the activity of phosphokinase GSK-3 尾 -AKTERK. However, the decrease in the total amount of phosphatase PP2A may be the main reason. The single nasal insulin administration did not reverse the excessive phosphorylation of the Thr205 site of tau protein induced by propofol. Conclusion: single nasal administration of insulin can not significantly protect propofol related abnormal changes. The use of insulin as a protective measure for postoperative cognitive dysfunction needs further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.16

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