番茄红素对转基因果蝇模型神经保护作用的研究

发布时间：2018-03-09 04:09

本文选题：番茄红素　切入点：果蝇　出处：《中国现代医学杂志》2014年35期 　论文类型：期刊论文

【摘要】：目的研究番茄红素对β-淀粉样蛋白(Aβ)42转基因果蝇的神经保护作用。方法将实验果蝇随机分为不同浓度干预组、二甲基亚砜(DMSO)溶剂对照组、模型组和正常果蝇对照组,喂养至果蝇死亡。记录各组果蝇的寿命和运动爬行的结果以及测定果蝇体内超氧化物歧化酶(SOD)、胆碱酯酶活性及脑组织病理变化。结果番茄红素能延长Aβ42转基因果蝇的寿命,改善它的爬行能力,能增强其体内的SOD活性,胆碱酯酶活性受到抑制。同时番茄红素能减少模型果蝇脑组织病变空泡形成。结论番茄红素对Aβ42转基因果蝇具有神经保护作用,其机制可能是通过抗氧化作用来实现的。
[Abstract]:Objective to study the neuroprotective effect of lycopene on transgenic Drosophila melanogaster with 尾 -amyloid protein A 尾 42. Methods Drosophila melanogaster was randomly divided into three groups: different concentration intervention group, DMSO solvent control group, model group and normal Drosophila control group. After feeding to the death of Drosophila melanogaster, the results of life span and movement crawling in each group were recorded, and the activity of superoxide dismutase (SOD), cholinesterase (cholinesterase) and pathological changes of brain tissue were measured. The results showed that lycopene could prolong the life span of A 尾 42 transgenic Drosophila. Improving its crawling ability can enhance the activity of SOD and inhibit the activity of cholinesterase. At the same time, lycopene can reduce the vacuole formation in the brain lesion of Drosophila melanogaster. Conclusion lycopene has neuroprotective effect on A 尾 42 transgenic Drosophila. The mechanism may be through antioxidant action.
【作者单位】：桂林医学院附属医院;
【基金】：国家自然科学基金(No:81260496) 广西壮族自治区卫生厅自筹课题(No:Z2012419)
【分类号】：R749.16

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