熊果酸改善慢性温和不可预知应激小鼠抑郁行为的研究

发布时间：2018-03-13 06:09

本文选题：熊果酸　切入点：抗抑郁作用　出处：《现代预防医学》2017年14期 　论文类型：期刊论文

【摘要】：目的研究熊果酸改善慢性温和不可预知应激抑郁模型(CUMS)小鼠行为及下丘脑-垂体-肾上腺轴(HPA)轴功能失调的作用。方法 ICR小鼠接受温和不可预知刺激,28 d后经糖水偏好实验评价模型,分组:CUMS组、熊果酸干预组(灌胃,5、10、20 mg/kg)和氟西汀阳性对照组(灌胃,5 mg/kg),同时设置正常组。给药持续21 d,最后1周进行糖水偏好实验、矿场试验和强迫游泳实验,评价熊果酸对CUMS小鼠抑郁行为的影响,并检测血清促肾上腺皮质激素和皮质酮及海马糖皮质激素受体蛋白水平的变化,评价HPA轴功能。结果熊果酸增加CUMS小鼠体重(P0.01,P0.001),提高小鼠糖水偏好值(P0.05,P0.01)和自主运动时间(水平:P0.01,P0.001;垂直:P0.01,P0.01),并降低强迫游泳不动时间(P0.05,P0.01)。熊果酸还能降低血清促肾上腺皮质激素(P0.05)和皮质酮水平(P0.05),增加海马糖皮质激素受体蛋白表达(P0.01)。氟西汀也能抑制CUMS小鼠的抑郁行为和HPA轴的异常变化,其作用效果与高剂量熊果酸的相当。结论熊果酸具有抗抑郁作用,其机制可能与抑制HPA轴的亢进相关。
[Abstract]:Objective to study the effects of ursolic acid on the behavior and dysfunction of hypothalamus-pituitary-adrenal axis in chronic mild and unpredictable stress depression mice. Methods ICR mice were treated with mild unpredictable stimulation for 28 days. Experimental Evaluation Model of Sugar Water preference, They were divided into two groups: 1 / CUMS group, ursolic acid intervention group (10 ~ 10 mg 路kg ~ (-1) 路kg ~ (-1)) and fluoxetine positive control group (5 mg 路kg ~ (-1) 路kg ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1)), respectively. To evaluate the effect of ursolic acid on depressive behavior in CUMS mice and to detect the changes of serum corticotropin and corticosterone and hippocampal glucocorticoid receptor protein. Results ursolic acid increased the body weight of CUMS mice and increased the sugar water preference value (P0.05, P0.01) and autonomous exercise time (horizontal: P0.01P0.01P0.001; Vertical: P0.01P0.01P0.01A, and decreased the immobility time of forced swimming P0.05P0.01. ursolic acid also decreased the serum adrenal cortex stimulating time (P0.05P0.01a). P0.05) and corticosterone increased the expression of glucocorticoid receptor protein in hippocampus. Fluoxetine also inhibited depressive behavior and abnormal changes of HPA axis in CUMS mice. Conclusion ursolic acid has antidepressant effect, and its mechanism may be related to the inhibition of hyperactivity of HPA axis.
【作者单位】：山东省临沂市沂水中心医院;
【分类号】：R749.4

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