抑郁症大鼠海马体积异常的形态学探讨

发布时间：2018-03-13 15:03

  本文选题：抑郁症　切入点：海马　出处：《中风与神经疾病杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的观察抑郁症模型大鼠海马神经元形态结构改变及凋亡、自噬的变化,探讨抑郁症海马体积异常的机制。方法选用雄性成年SD大鼠,随机分为正常对照组和模型组,通过给予不可预见慢性温和应激建立抑郁症模型;采用尼氏染色观察海马神经元形态变化,流式细胞术检测海马神经元凋亡,Western blotting检测自噬相关蛋白LC-3和Beclin 1。结果与对照组比较,模型组海马体积萎缩,神经元数量减少,细胞凋亡率增高(P0.05)。与对照组相比,模型组LC-3Ⅱ蛋白和LC-3Ⅱ/LC-3Ⅰ比值增高,Beclin 1相对表达增高(P0.05)。结论抑郁症大鼠海马凋亡和自噬增强,可能是海马体积异常的原因之一。
[Abstract]:Objective to observe the morphological changes, apoptosis and autophagy of hippocampal neurons in rats with depression, and to explore the mechanism of abnormal hippocampal volume. Methods male adult Sprague-Dawley rats were randomly divided into normal control group and model group. Depression model was established by giving unpredictable chronic mild stress, morphological changes of hippocampal neurons were observed by Nissl staining, apoptosis of hippocampal neurons was detected by flow cytometry, autophagy associated protein LC-3 and Beclin 1 were detected by flow cytometry. Compared with the control group, the LC-3 鈪，

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