丙戊酸孤独症大鼠脑中经典Wnt信号通路的变化

发布时间：2018-03-13 21:14

本文选题：孤独症　切入点：Wnt信号通路　出处：《中国病理生理杂志》2014年08期 　论文类型：期刊论文

【摘要】：目的:探讨经典Wnt信号通路在孤独症发病中的作用。方法:利用丙戊酸孤独症大鼠模型,检测经典Wnt信号通路信号分子在孤独症大鼠前额叶皮质及海马脑区的表达。Western blotting法检测糖原合成酶激酶3β(glycogen synthase kinase 3β,GSK-3β)、磷酸化GSK-3β、β-catenin和磷酸化β-catenin表达,半定量RT-PCR法检测GSK-3β、β-catenin、c-Myc和cyclin D1 mRNA表达。结果:与对照组相比,在丙戊酸孤独症大鼠前额叶皮质及海马脑区,失活的GSK-3β磷酸化表达显著增加,抑制性的β-catenin磷酸化表达显著减少;GSK-3βmRNA表达减少,β-catenin mRNA表达增加,下游c-Myc和cyclin D1 mRNA表达增加。结论:前额叶皮质及海马中经典Wnt信号通路活性增加可能促进了机体对孤独症的易感性。
[Abstract]:Objective: to investigate the role of classical Wnt signaling pathway in the pathogenesis of autism. Expression of classical Wnt signaling molecules in prefrontal cortex and hippocampus of autistic rats. Western blotting method was used to detect the expression of glycogen synthase kinase 3 尾 -GSK-3 尾, phosphorylated GSK-3 尾, 尾 -catenin and phosphorylated 尾 -catenin. The expression of GSK-3 尾, 尾 -cateninine c-Myc and cyclin D1 mRNA was detected by semi-quantitative RT-PCR. Results: compared with the control group, inactivated GSK-3 尾 phosphorylation was significantly increased in prefrontal cortex and hippocampal area of rats with valproic acid autism. The inhibition of 尾 -catenin phosphorylation significantly decreased the expression of GSK-3 尾 mRNA, increased the expression of 尾 -catenin mRNA, and increased the expression of c-Myc and cyclin D1 mRNA downstream. Conclusion: the increased activity of classical Wnt signaling pathway in prefrontal cortex and hippocampus may promote susceptibility to autism.
【作者单位】：新乡医学院解剖教研室;复旦大学上海医学院人体解剖与组织胚胎学系;九江学院基础医学院生理学与病理生理学教研室;
【基金】：国家自然科学基金资助项目(No.81301174;No.81260211) 新乡医学院博士科研启动基金资助项目(No.505003)
【分类号】：R749.94

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