NINJ2基因多态性与血管性认知功能障碍的相关性研究

发布时间：2018-03-14 23:03

本文选题：血管性认知障碍　切入点：NINJ2基因　出处：《青岛大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:探讨神经损伤诱导蛋白(NINJ2)基因rs11833579、rs12425791位点多态性与血管性认知功能障碍(Vascular cognitive impairment,VCI)的相关性。方法:采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)对112例VCI患者(VCI组)、108例脑梗死后无认知功能障碍患者(NCI组)及113例健康者(对照组)的NINJ2 rs11833579、rs12425791位点进行基因型检测,比较基因型和等位基因频率在各组是否存在差异并进行关联性分析。结果:(1)rs11833579位点:三组间基因型、等位基因分布差异均无统计学意义(x2=1.942,P0.05;X2=1.343,P0.05);多项logistic回归校正危险因素后,VCI组及NCI组AA+AG基因型对患病风险无明显影响。(2)rs12425791位点:三组基因型、等位基因分布差异有统计学意义(X2=27.036,P0.05;X2=19.816,P0.05);多项logistic回归校正危险因素后,VCI组及NCI组AA+AG基因型与VCI、脑梗死的发病呈相关关系(OR=1.785,95%CI 1.008~3.159,P0.05;OR=1.864,95%CI1.061~3.274,P0.05)。(3)三组间性别、年龄、吸烟、血脂TG、TC水平比较,差异没有统计学意义(P0.05)。VCI组高血压病和糖尿病的发病率显著高于对照组(P0.05),血脂高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平和对照组比较,差异有统计学意义(P0.05)。结论:1.NINJ2基因rs12425791位点的多态性与VCI有相关性,该位点对VCI的影响可能与脑损伤修复有关。2.本研究VCI组与对照组相比,高血压病和糖尿病的发病率、血脂LDL水平和HDL水平差异有统计学意义,提示高血压、糖尿病、高LDL水平可能是VCI的危险因素。
[Abstract]:Objective: to investigate the association between rs11833579 (rs12425791) polymorphism and Vascular cognitive impairment (VCII) of nerve injury inducible protein nin J22.Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used in 112 patients with VCI. The genotypes of NINJ2 rs11833579 (rs12425791) were detected in 108 patients with no cognitive impairment after cerebral infarction and 113 healthy subjects (control group). The genotypes and allelic frequencies were compared among the three groups and the correlation analysis was carried out. Results: 1: 1 rs11833579: genotypes among the three groups, There was no significant difference in allelic distribution between VCI group and NCI group (P 0.05 rs12425791), there was no significant difference in allelic distribution between VCI group and NCI group, and there was no significant difference in alleles distribution between VCI group and NCI group (P 0.05 rs12425791 locus: three genotypes), and there was no significant difference in AA AG genotype between VCI group and NCI group after adjustment of risk factors by multiple logistic regression. There was a significant difference in allele distribution among the three groups: sex, age, smoking, blood lipids and TGTC levels in VCI group and NCI group after adjustment for risk factors. The incidence of cerebral infarction was related to the incidence of CI 1.785Cl 1.78595 CI 1.0083.159P0.05OR1.86495 CI1.0613.274P0.05.3The three groups were compared with each other in sex, age, smoking, and TGTC levels. There was no significant difference in the incidence of hypertension and diabetes in the group of P0.05. VCI and compared with that in the control group. The levels of HDLN and LDL were significantly higher in the VCI group than in the control group. Conclusion: 1. The polymorphism of the rs12425791 locus of NINJ2 gene is associated with VCI, and the effect of this locus on VCI may be related to the repair of brain injury. 2. The incidence of hypertension and diabetes mellitus in the VCI group was higher than that in the control group. There were significant differences between LDL and HDL levels, suggesting that hypertension, diabetes and high LDL levels might be risk factors for VCI.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R749.13

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