α1整合素活化在β淀粉样蛋白诱导神经细胞死亡中的作用

发布时间：2018-03-15 10:03

  本文选题：整合素　切入点：黏着斑激酶　出处：《中国老年学杂志》2017年18期 　论文类型：期刊论文

【摘要】：目的探讨神经母细胞瘤细胞(SH-SY5Y)中β淀粉样蛋白(Aβ)诱导丝裂原活化蛋白激酶(MAPK)信号通路活化的上游影响因素。方法选择SH-SY5Y为靶细胞,首先用MAPK信号通路上游抑制剂处理细胞,待细胞培养1 h之后,加入Aβ42纤维聚集体,细胞培养24 h后进行检测。首先采用甲氮甲唑蓝(MTT)法测定了细胞的存活率,然后利用Western印迹检测磷酸化细胞外信号调节蛋白激酶(p-ERK)和ERK的蛋白表达水平,从而检测MAPK信号通路的激活水平。结果用锯鳞血抑肽或α1整合素蛋白封闭性抗体可以有效抑制Aβ引起的细胞死亡,并且阻止Aβ成纤维诱导MAPK的激活水平的提高(P0.05)。用α1和β1整合素蛋白封闭抗体同时作用细胞时,得到了相似的结果。但是如果只用β1整合素蛋白封闭抗体单独作用细胞时,不能阻止Aβ成纤维诱导细胞死亡和MAPK的激活水平的提高。结论α1整合素,α1和β1整合素复合体是Aβ诱导SH-SY5Y细胞中MAPK信号通路激活从而介导细胞死亡的重要因素。推测α1整合素蛋白和α1、β1复合体可以作为治疗性干扰Aβ信号通路的靶点。
[Abstract]:Objective to investigate the upstream effects of 尾 -amyloid protein A 尾 on mitogen-activated protein kinase (MAPK) signal pathway activation in neuroblastoma cell line SH-SY5Y.Methods SH-SY5Y was selected as target cell and MAPK signal pathway upstream inhibitor was used to treat cells. After the cells were cultured for 1 hour, A 尾 42 fiber aggregates were added, and the cells were cultured for 24 h. The survival rate of the cells was determined by methazolyl methazolyl methionine (MTT) method. Then Western blot was used to detect the expression of phosphorylated signal regulated protein kinase p-ERK (p-ERK) and ERK. The activation level of MAPK signaling pathway was detected. Results A 尾 -induced cell death could be effectively inhibited by anti-peptide or 伪 1 integrin protein blocking antibody. The same results were obtained when 伪 1 and 尾 1 integrin protein blocked the cells simultaneously. However, if only 尾 1 integrin protein blocked the cells, but only 尾 1 integrin protein blocked the cells alone. Conclusion 伪 _ 1 integrin, 伪 _ 1 and 尾 _ 1 integrin complexes are important factors for the activation of MAPK signaling pathway in A 尾 -induced SH-SY5Y cells and thus mediates cell death. 伪 1 integrin protein and 伪 1, 尾 1 complex can be used as targets for therapeutic interference of A 尾 signaling pathway.
【作者单位】： 郑州大学附属南阳医院南阳市中心医院神经外科;
【分类号】：R749.16
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本文编号：1615543