骨髓间充质干细胞对大鼠脑创伤后认知功能的影响

发布时间：2018-03-23 03:02

本文选题：BMSCs　切入点：创伤性脑损伤　出处：《中国免疫学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:探讨大鼠在脑创伤后,骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)移植对大鼠认知功能的影响。方法:体外分离、培养雄性3周龄SD大鼠BMSCs传至3代备用。选取60只SD雌性大鼠随机分为3组(n=20)、对照组(Control group)、模型组(Model group)、BMSCs治疗组(BMSCs treatment group)。使用脑皮质挫伤撞击仪制作大鼠脑创伤模型,分别在模型建立成功后的第1、7、14、28天BMSCs治疗组给予BMSCs(1×106ml-1)干预治疗。另取20只大鼠行水迷宫测试,每组划分为伤后6、7、8及9 d四个时间点。HE染色法检测脑组织形态学变化;干湿比重法检测脑组织水含量;Morris检测法检测大鼠的空间学习记忆能力;神经运动功能评分检测分析神经功能状态;矿场实验检测大鼠的空间学习记忆能力。结果:干湿比重法检测结果显示与对照组相比,模型组均明显高于对照组(P0.05),治疗组在每个时间点的水含量与模型组相比又显著的下降;水迷宫检测结果,模型组在伤后第8天和第9天搜索安全岛潜伏期明显要比对照组时间增加,差异有统计学意义(P0.05)。治疗组在伤后第8天以及第9天搜索安全岛潜伏期要比对照组有显著的缩短;神经功能评分结果显示,与对照组比,模型组的大鼠在后肢抓持反射、触须诱发前肢定位以及侧向垫步的测试中都有不正常的表现,导致功能评分降低,具有统计学意义(P0.05),治疗组和模型组比,各项的功能评分有显著的升高。结论:通过对行为学相关实验的检测,数据显示在大鼠脑创伤后BMSCs能够在一定程度上对神经认知功能起到恢复的作用。
[Abstract]:Objective: to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on cognitive function in rats after brain trauma. Male 3-week-old Sprague-Dawley rats (BMSCs) were cultured for 3 generations. Sixty female SD rats were randomly divided into three groups: control group, control group and model group. Brain injury model was established by cortical contusion impactor. The rats in the BMSCs group were treated with BMSCs(1 脳 106ml-1 on the 28th day after the establishment of the model. The other 20 rats were tested by water maze test. Each group was divided into 4 time points after injury. The morphological changes of brain tissue were detected by HE staining method. The content of water in brain tissue was measured by dry-wet specific gravity method and the spatial learning and memory ability of rats was detected by Morris method. Results: compared with the control group, the model group was significantly higher than the control group (P 0.05), and the water content of the treatment group at each time point was significantly lower than that of the model group. The results of water maze test showed that the incubation period of searching safety island in model group was significantly longer than that in control group on the 8th and 9th day after injury. The latency of searching for safe islands in the treatment group was significantly shorter than that in the control group on the 8th and 9th day after injury, and the neurological function score showed that compared with the control group, the rats in the model group had clasped reflexes in the hind limbs. The antebrachial localization induced by tentacles as well as the lateral pad steps showed abnormal manifestations, resulting in a decrease in the functional score, with statistical significance (P0.05), compared with the treatment group and the model group. Conclusion: the data show that BMSCs can recover neurocognitive function to some extent after traumatic brain injury.
【作者单位】：承德医学院;
【分类号】：R749.12

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