BMSC向神经细胞分化诱导后上清液对MCI大鼠治疗作用的实验研究

发布时间：2018-03-29 16:04

本文选题：认知功能障碍　切入点：骨髓间充质干细胞　出处：《中西医结合心脑血管病杂志》2012年06期

【摘要】：目的探讨3代、4代、5代大鼠骨髓间充质干细胞(BMSC)向神经细胞诱导后12h上清液对认知功能障碍大鼠的治疗作用。方法行Morris水迷宫行隐蔽平台获得实验对比各组间注射上清液前后大鼠潜伏期,免疫组织化学法对比各组间注射前后海马周期蛋白依赖性激酶5(CDK5)表达情况。结果 Morris水迷宫隐蔽平台获得实验,除3代组与5代组之间差异无统计学意义外,其他任何两组间的差异均有统计学意义,3代与5代上清液较4代效果明显。大鼠脑组织CDK5表达除3代组与5代组间差异无统计学意义外,余各组间差异均有统计学意义,3代与5代上清液组较4代CDK5表达阳性率低。结论 3代、4代、5代大鼠BMSC向神经细胞诱导后12h上清液对认知功能障碍大鼠有治疗作用,3代与5代作用较4代明显。
[Abstract]:Objective to investigate the therapeutic effect of bone marrow mesenchymal stem cells (BMSCs) on rats with cognitive impairment 12 h after induction of neural cells. Methods Morris water maze was used to obtain the experimental results of experimental comparison. The incubation period of rats before and after supernatant injection, Immunohistochemical method was used to compare the expression of cyclin dependent kinase 5 (CDK5) in hippocampus before and after injection. Results the Morris water labyrinth covert platform was obtained, except that there was no significant difference between the 3rd generation group and the 5th generation group. The difference between the other two groups was statistically significant. The expression of CDK5 in the brain tissue of the rats was significantly higher than that of the fourth generation, except that there was no significant difference between the third generation group and the fifth generation group, and there was no significant difference between the third generation group and the fifth generation group in the expression of CDK5. The positive rate of CDK5 expression in the third and fifth generation supernatant groups was lower than that in the fourth generation group. Conclusion the supernatant of BMSC in the third and fifth generation rats can be used to treat the rats with cognitive impairment 12 hours after the induction of BMSC into nerve cells. The effects of three and five generations were more obvious than those of the fourth generation.
【作者单位】：山西医科大学第二医院;
【分类号】：R749.16

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