高压氧对慢性疲劳综合征大鼠影响的实验研究

发布时间：2018-03-31 04:11

本文选题：慢性疲劳综合症　切入点：高压氧　出处：《第二军医大学》2012年硕士论文

【摘要】：慢性疲劳综合征(Chronic Fatigue Syndrome，CFS)是一组以长期极度疲劳为主要表现的全身性综合征。随着现代生活节奏的加快，慢性疲劳综合征（chronic fatiguesyndrome，CFS）的发病率日益增高，严重影响了人们生活。我国的CSF发病率为10%～20%，但增长速度惊人。由于CFS的病因还不明确，因此缺乏特异的动物模型和治疗手段。高压氧治疗可以提高血氧含量，改善细胞代谢，调节身体各器官功能，可缓解HIV感染和剧烈运动等原因引起的疲劳。 1目的通过建立CFS动物模型，并予以高压氧治疗，观察动物的行为学改变以及甲状腺激素（TSH、T3、T4）、性激素（FSH、LH）、血管内皮素等指标的改变，评价高压氧治疗应用于CFS治疗的价值。 2．方法 2.1将SD大鼠随机分为空白对照组、高压氧对照组、CFS模型组、CFS高压氧组及CFS休息组，采用负重力竭游泳的方法建立CFS模型。评价各组大鼠的一般情况、力竭游泳时间、爬杆实验、旷场实验的完成情况。 2.2采用放射免疫法检测血清甲状腺激素（TSH、T3、T4）水平 2.3采用放射免疫法检测血管内皮功能:血浆内皮素-1（endothelin-1，ET-1）、一氧化氮（nitric oxide，NO）及血管性假血友病因子（von wille-brand Factor，vWF）的影响。 2.4采用放射免疫法检测性激素（FSH、LH） 3．结果 3.1CFS模型组大鼠与空白对照组比较，大鼠一般情况变差，体重减轻，力竭游泳时间缩短，爬杆时间缩短，旷场实验中央格滞留时间明显延长，跨格次数、修饰时间及修饰次数明显减少（7项指标）。CFS高压氧组与CFS模型组比较，大鼠的上述7项指标明显好转（P0.05）；但CFS高压氧组与空白对照组比较，大鼠7项指标仍恢复不理想（P0.05）。CFS休息组与CFS模型组比较，7项指标无明显改善（P0.05）。 3.2CFS模型组较空白对照组血清三碘甲腺原氨酸（T3）明显降低（P0.05）；CFS高压氧组T3水平较CFS模型组有所恢复（P0.05），具有统计学意义；CFS高压氧组T3较空白对照组、高压氧对照组仍偏低（P0.05）；5组大鼠的TSH、T4水平无明显差异，P0.05。 3.3CFS模型组较空白对照组血清ET-1、vWF升高（P0.05），而NO降低（P0.05）；CFS高压氧组以上指标较CFS模型组呈反向变化（P0.05），具有统计学意义；CFS高压氧组以上指标较空白对照组、高压氧对照组无差异（P0.05）。 3.4CFS高压氧组促性腺激素（FSH、LH）水平较CFS模型组、CFS休息组有明显恢复，具有统计学意义。空白对照组与高压氧对照组激素水平无显著差异。 4．结论 4.1慢性疲劳综合征大鼠单纯休息不能缓解，高压氧治疗可缓解其症状，正常大鼠行高压氧治疗对其行为学没有明显影响。 4.2慢性疲劳综合征大鼠血清T3下降，，经高压氧治疗后T3水平较前有所恢复，说明高压氧对慢性疲劳综合征有积极作用。 4.3慢性疲劳综合征大鼠存在血管内皮功能障碍，高压氧治疗能够改善模型动物的血管内皮功能。 4.4慢性疲劳综合征大鼠高压氧治疗后促性腺激素（FSH、LH）较前明显恢复，正常动物行高压氧治疗对促性腺激素（FSH、LH）水平无影响
[Abstract]:Chronic fatigue syndrome (Chronic Fatigue, Syndrome, CFS) is a systemic syndrome in a group of long-term fatigue mainly. With the fast pace of modern life, chronic fatigue syndrome (chronic, fatiguesyndrome, CFS) the incidence is increasing, serious impact on people living in China. The incidence of CSF was 10% to 20%, but the growth rate is amazing. Due to the etiology of CFS is not clear, so the lack of animal model and specific treatment.
Hyperbaric oxygen therapy can improve blood oxygen content, improve cell metabolism, regulate the function of various organs of the body, and relieve the fatigue caused by HIV infection and severe exercise.
1 purposes
By establishing CFS animal model and hyperbaric oxygen therapy, we observed animal behavior changes, TSH T3 (T4), sex hormones (FSH, LH), et et al, and evaluated the value of hyperbaric oxygen therapy in CFS treatment.
2. method
2.1 SD rats were randomly divided into blank control group, hyperbaric oxygen control group, CFS model group, CFS hyperbaric oxygen group and CFS rest group. CFS model was established by weight-bearing exhaustive swimming. The general condition, exhaustive swimming time, pole climbing test and open field experiment were evaluated.
2.2 the levels of serum thyroid hormone (TSH, T3, T4) were detected by radioimmunoassay
2.3, the effects of plasma endothelin -1 (endothelin-1, ET-1), nitric oxide (NO) and von Willebrand factor (von wille-brand Factor, vWF) were detected by radioimmunoassay.
2.4 FSH (LH) was detected by radioimmunoassay.
3. results
With the blank group rats 3.1CFS model control group, rats in general variation, weight loss, exhaustive swimming time, climbing pole time, residence time of the open field test in central square was prolonged, the grid crossing frequency, modification time and modification times were significantly reduced (7 indicators).CFS hyperbaric oxygen group and CFS compared to the model group, the rats of the 7 indicators improved significantly (P0.05); but CFS hyperbaric oxygen group compared with the control group, 7 rats were still not ideal recovery index (P0.05).CFS rest group comparing with CFS model group, 7 indexes had no obvious improvement (P0.05).
The 3.2CFS model group compared with blank control group, three serum triiodothyronine (T3) decreased significantly (P0.05); CFS hyperbaric oxygen group T3 levels compared with CFS group (P0.05) has been restored, with statistical significance; CFS T3 in HBO group than the control group, hyperbaric oxygen group (P0.05) is still low; 5 rats in group TSH, no significant differences in the level of T4, P0.05.
The 3.3CFS model group compared with blank control group, serum ET-1 and vWF increased (P0.05), and NO decreased (P0.05); CFS hyperbaric oxygen group compared with CFS group the above indexes showed the reverse changes (P0.05), with statistical significance; CFS index above the hyperbaric oxygen group compared with blank control group, no difference in hyperbaric oxygen group (P0.05).
The level of FSH (LH) in 3.4CFS hyperbaric oxygen group was significantly higher than that in CFS model group and CFS rest group, and there was statistical significance. There was no significant difference in hormone level between blank control group and hyperbaric oxygen control group.
4. conclusion
4.1, chronic fatigue syndrome rats can not alleviate rest alone. Hyperbaric oxygen therapy can relieve their symptoms.
4.2 the serum T3 of rats with chronic fatigue syndrome decreased and the level of T3 recovered after hyperbaric oxygen therapy. It indicated that hyperbaric oxygen had a positive effect on chronic fatigue syndrome.
4.3 the rats with chronic fatigue syndrome have vascular endothelial dysfunction, and hyperbaric oxygen therapy can improve the vascular endothelial function of model animals.
4.4, FSH, LH in chronic fatigue syndrome rats were significantly restored after hyperbaric oxygen treatment. No significant difference was found in the level of gonadotrophin (FSH, LH) in normal animals.

【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.9

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