丁苯酞对血管性痴呆大鼠海马CA1区脑源性神经营养因子表达的影响

发布时间：2018-03-31 10:45

本文选题：血管性痴呆　切入点：海马　出处：《吉林大学学报(医学版)》2017年05期

【摘要】：目的:观察丁苯酞(NBP)对血管性痴呆(VD)大鼠海马CA1区脑源性神经营养因子(BDNF)表达的影响,探讨丁苯酞对VD的保护作用。方法:将80只健康Wistar大鼠按随机区组法分为假手术组、NBP对照组(假手术+NBP注射剂)、VD组(VD模型)和NBP处理组(VD模型+NBP注射剂),每组20只,每组又分为4个亚组,即术后1、2、4和8周组,每个亚组5只。永久性双侧颈总动脉结扎法制备VD大鼠模型。NBP对照组和NBP处理组大鼠腹腔注射NBP注射剂5mg·kg~(-1)·d~(-1),连续给药7d。假手术组和VD组大鼠每次腹腔注射4mL生理盐水,连续注射7d。各组大鼠在术后各时间点(1、2、4和8周)留取海马组织,应用实时定量PCR和免疫组织化学法检测各组大鼠海马CA1区BDNF表达水平。结果:定时定量PCR法,术后2、4和8周,VD组大鼠海马BDNF mRNA表达水平明显高于假手术组(P0.05),术后4和8周,NBP处理组大鼠海马BDNF mRNA表达水平明显高于VD组(P0.05);免疫组织化学法,VD组大鼠4周时BDNF表达水平明显高于假手术组(P0.05),术后8周时NBP组大鼠CA1区BDNF蛋白表达水平明显高于VD组(P0.05)。结论:VD模型大鼠海马CA1区神经元在遭受缺血损伤后,BDNF反应性表达增加,而NBP则可明显提高VD大鼠海马CA1区BDNF的表达,发挥神经保护作用。
[Abstract]:Aim: to observe the effect of butyphthalide (NBP) on the expression of brain-derived neurotrophic factor (BDNF) in CA1 area of vascular dementia rats. To investigate the protective effect of butyphthalide on VD. Methods: 80 healthy Wistar rats were randomly divided into two groups: sham operation group (n = 20) and NBP treated group (n = 20). Each group was subdivided into 4 subgroups, namely, 1, 2, 4 and 8 weeks after operation. Five rats in each subgroup were used to establish VD rat model by permanent bilateral common carotid artery ligation. Rats in the control group and the NBP treatment group were injected intraperitoneally with NBP injection 5mg KGG -1) for 7 days. Rats in sham operation group and VD group were intraperitoneally injected with 4mL saline. After 7 days of continuous injection, the hippocampal tissue was taken from the rats of each group at each time point. The expression of BDNF in the hippocampal CA1 was detected by real-time quantitative PCR and immunohistochemical method. Results: the expression of BDNF in the hippocampus of the rats in each group was measured by quantitative PCR method. The expression of BDNF mRNA in hippocampus of VD group was significantly higher than that of sham operation group at 2 and 8 weeks postoperatively, and the expression of BDNF mRNA in hippocampus of VD group was significantly higher than that of VD group at 4 and 8 weeks after operation, and the expression of BDNF in VD group was significantly higher than that in VD group at 4 weeks and 8 weeks after operation, and the expression of BDNF mRNA in VD group was significantly higher than that in VD group at 4 weeks after operation. The expression of BDNF protein in CA1 was significantly higher in NBP group than that in VD group at 8 weeks after operation. Conclusion the reactive expression of BDNF in hippocampal CA1 neurons of rats with NBP was increased after ischemia injury. NBP could significantly increase the expression of BDNF in hippocampus CA1 of VD rats and play a neuroprotective role.
【作者单位】：吉林大学第二医院神经内科;
【基金】：国家自然科学基金资助课题(31372267,81500953) 吉林省科技厅科技发展计划项目资助课题(20150520143JH)
【分类号】：R749.13

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