急性应激期大鼠学习敏感化的改变及其对PTSD样症状形成的预测作用

发布时间：2018-04-02 09:25

本文选题：创伤后应激障碍　切入点：急性应激期　出处：《湖南师范大学》2012年硕士论文

【摘要】：目的：采用不同应激方式与强度建立创伤后应激障碍动物模型,并探讨其应激敏感化中学习能力的改变以及这种学习能力敏感性是否对创伤后应激障碍症状的形成产生影响。方法：实验一,选取72只雄性SD大鼠,采用福尔马林注射加足部电击、单程长时应激模式建立不同创伤后应激障碍动物模型,采用惊听觉反应箱、高架十字迷宫实验、社交趋避等测试方法对创伤后应激障碍大鼠的高唤醒症状、回避症状和社交回避症状进行行为测验,以检验创伤后应激障碍动物模型的表面效度。实验二,选取48只雄性SD大鼠,采用福尔马林注射加足部电击建立创伤后应激障碍动物模型,采用惊听觉反应箱及抑制性回避测试测试大鼠急性应激期非联想性学习(中性、负性刺激)的改变；采用高架十字迷宫实验、社交趋避等方法测试创伤后应激障碍大鼠的回避和社交回避症状行为,以检验急性应激期非联想性学习的改变是否能对PTSD症状进行预测。实验三,选取48只雄性SD大鼠,采用福尔马林注射加足部电击建立创伤后应激障碍动物模型,采用食物位置偏爱方法测试大鼠急性应激期非联想性学习(正性)的改变；采用高架十字迷宫实验、社交趋避等方法测试创伤后应激障碍大鼠的回避和社交回避症状行为,以检验急性应激期正性刺激学习的改变是否能对PTSD症状进行预测。结果：实验一：不同应激组与对照组之间均存在差异,福尔马林足部电击组与对照组间差异显著。高唤醒症状中福尔马林足部电击组与对照组之间存在显著性差异(t=53.82,p=0.001)；回避症状行为测试显示,福尔马林组在开放臂的时间(p=0.009)和次数(t=0.185,p=0.008)显著少于对照组；社交回避症状中福尔马林足部电击组进入社交区域的时间(t=23.00,p=0.019)和次数(t=1.962,p=0.009)低于对照组。实验二：急性应激前期对与创伤无关刺激的反应,应激组低于对照组,但二者之间没有显著性差异(t=0.477,p=0.638)；急性应激后期,应激组与对照组存在着显著的差异(t=3.126,p=0.005)。急性应激前期负性学习能力的测试显示,应激组与对照组相比进入微弱电击侧的潜伏期更长,但是二者差异不显著(t=-1.264,p=0.230)；急性应激后期,对照组与应激组存在显著性差异(t=-2.337,p=0.032)；急性应激后期与创伤无关刺激行为反应结果(90dB)与回避行为症状结果显著性相关(r=0.455,p=0.026)；急性应激后期负性学习能力测试值与社会回避行为测试数据高相关(r=-0.553,p=0.029)。实验三：急性应激期对照组与应激组在正性学习能力上没有显著性差异(t=-1.092,p=0.316)；急性应激前期正性学习能力与回避症状(r=0.003,p=0.991)、社会回避症状(r=-0.107,p=0.645)之间相关不显著；急性应激后期正性学习能力与回避症状(r=0.009,p=0.969)、社会回避症状(r=-0.138,p=0.552)之间相关不显著。结论：1、福尔马林足部电击模型是具有较高表面效度的PTSD动物模型。2、PTSD大鼠在急性应激期对与创伤无关的刺激反应随时间而增强。3、PTSD大鼠在急性应激期负性刺激学习敏感性随时间不断增强。4、PTSD大鼠在急性应激期对正性刺激的学习敏感性与正常组大鼠之间没有显著性差异。5、急性应激后期对低强度与创伤无关刺激的反应能够对PTSD回避症状进行预测。6、急性应激后期对负性刺激学习敏感性能够对PTSD社交回避症状进行预测。
[Abstract]:Objective : To establish an animal model of post - traumatic stress disorder with different stress methods and intensity , and to explore the change of learning ability in stress - sensitivity and whether the sensitivity of learning ability affects the formation of post - traumatic stress disorder symptoms .

Methods : One hundred and seventy - two male SD rats were selected and 72 male SD rats were selected to establish animal models of post - traumatic stress disorder by using formalin injection and foot shock , single - pass long - time stress model , and the behavioral tests were conducted on the high - arousal symptoms , avoidance symptoms and social avoidance symptoms of post - traumatic stress disorder rats by using the test methods such as shock response box , elevated cross maze test and social avoidance , to test the surface validity of the animal model of post - traumatic stress disorder .

In the experiment , 48 male SD rats were selected , and the animal model of post - traumatic stress disorder was established by using formalin injection and foot electric shock , and the changes of non - associative learning ( neutral and negative stimulation ) in rats with acute stress period were tested by using a shock response box and an inhibitory avoidance test ;
In order to test whether the change of non - associative learning in acute stress period can predict the symptoms of posttraumatic stress disorder rats , the behavior of avoidance and social withdrawal symptoms of posttraumatic stress disorder rats were tested by elevated cross maze test and social avoidance method .

In the experiment , 48 male SD rats were selected , and the animal model of post - traumatic stress disorder was established by using formalin injection and heel electric shock , and the changes of non - associative learning ( positive ) in acute stress period of rats were measured by the method of food position preference .
The behavior of avoidance and social avoidance in rats with post - traumatic stress disorder was tested by elevated cross maze test , social avoidance , and so on .

Results : There was a significant difference between the different stress group and the control group . There was a significant difference between the formalin - foot electric shock group and the control group ( t = 53.82 , p = 0.001 ) .
The withdrawal symptoms behavior test showed that the time of the formalin group in the open arm ( p = 0 . 009 ) and the times ( t = 0.185 , p = 0.008 ) were significantly lower than that of the control group ;
The time ( t = 23.00 , p = 0.019 ) and the number of times ( t = 1.962 , p = 0 . 009 ) of the formalin - foot shock group in the social avoidance symptoms were lower than that of the control group .

Experiment 2 : There was no significant difference between the stress group and the control group ( t = 0.477 , p = 0.638 ) .
In the late period of acute stress , there was significant difference between the stress group and the control group ( t = 3.126 , p = 0.005 ) . Compared with the control group , the latent period was longer in the stress group than in the control group , but the difference was not significant ( t = - 1.264 , p = 0.230 ) .
In the late period of acute stress , there was significant difference between the control group and the stress group ( t = - 2.337 , p = 0.032 ) .
Results ( 90 dB ) were significantly correlated with the outcome of the response ( r = 0.455 , p = 0.026 ) .
The test value of negative learning ability after acute stress was highly correlated with the test data of social avoidance behavior ( r = - 0.553 , p = 0.029 ) .

Experiment 3 : There was no significant difference in positive learning ability between the control group and the stress group ( t = - 1.092 , p = 0.316 ) .
There was no significant correlation between positive learning ability and withdrawal symptoms in acute stress ( r = 0.003 , p = 0.9991 ) and social avoidance symptoms ( r = - 0.107 , p = 0.645 ) .
There was no significant correlation between positive learning ability and withdrawal symptoms ( r = 0 . 009 , p = 0.969 ) and social avoidance ( r = - 0.138 , p = 0.552 ) .

Conclusion : 1 . The model of foot electric shock of formalin is an animal model with high surface validity . In the acute stress period , the sensitivity of the rats to the acute stress is increased with the time .

【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.5

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