阿尔茨海默病患者血浆淀粉样变水平对血液物理指数的影响

发布时间：2018-04-08 20:11

本文选题：阿尔茨海默病　切入点：全血黏度　出处：《中国老年学杂志》2014年18期

【摘要】：目的探讨阿尔茨海默病(AD)患者血液物理指数与血浆淀粉样变水平之间的相关性。方法按照NINCDS-ADRDA标准临床诊断AD患者30例,年龄、性别与AD匹配的非痴呆健康对照组人群30例,硫磺素T(Thioflavin T,ThT)法测定血浆淀粉样变荧光强度,LBY-N6A血流变仪测定血浆全血黏度、血浆浓度、血沉、红细胞压积、全血高切相对指数、全血低切相对指数、血沉方程k值、红细胞聚集指数、全血低切还原黏度、全血高切还原黏度、红细胞刚性指数、红细胞变形指数TK十五项物理参数,SDS-PAGE电泳考马斯亮蓝蛋白染色结合SDS-PAGE电泳抗A抗体免疫反应,观察血浆Aβ蛋白的分布,相关分析血液物理指数与淀粉样变荧光强度。结果 AD患者血浆淀粉样变荧光强度和全血黏度(高切部分)明显高于对照组(P0.05),全血黏度值、红细胞比容、全血高切相对指数、全血低切相对指数与血浆淀粉样变水平呈正相关性。结论血浆物理指数全血黏度(高切部分)与血浆淀粉样变水平相关,改变血浆物理指数有可能有助于改善AD患者血液状态。
[Abstract]:Objective to investigate the correlation between blood physical index and plasma amyloidosis level in patients with Alzheimer's disease (AD).Methods 30 patients with AD were diagnosed according to NINCDS-ADRDA standard, 30 healthy controls were matched with AD by age and sex. Plasma whole blood viscosity and plasma concentration were measured by plasma amyloidosis fluorescence intensity and plasma plasma amyloidosis fluorescence intensity and plasma concentration by LBY-N6A hemorheometer.ESR, hematocrit, whole blood high shear relative index, whole blood low shear relative index, ESR equation k value, erythrocyte aggregation index, whole blood low shear reduction viscosity, whole blood high shear reduction viscosity, erythrocyte rigidity index,Erythrocyte deformability index (TK) was measured by SDS-PAGE gel electrophoresis with Coomassie brilliant blue protein staining combined with anti-A antibody immunoreaction by SDS-PAGE electrophoresis. The distribution of A 尾 protein in plasma was observed. The correlation analysis was made between the blood physical index and the intensity of amyloid fluorescence.Results the plasma amyloidosis fluorescence intensity and whole blood viscosity (high shear part) in AD patients were significantly higher than those in the control group (P 0.05), the whole blood viscosity, the specific volume of erythrocytes and the whole blood high shear relative index were significantly higher than those in the control group.There was a positive correlation between whole blood low shear relative index and plasma amyloidosis level.Conclusion the whole blood viscosity (high shear part) of plasma physical index is correlated with the level of plasma amyloidosis, and the change of plasma physical index may help to improve the blood status of AD patients.
【作者单位】：天津医科大学医学检验学院;天津医科大学总医院;
【基金】：国家自然科学基金(30973157)
【分类号】：R749.16

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