非线性动力学分析在重复经颅磁刺激对阿尔茨海默病大鼠学习记忆能力改善方面的应用

发布时间：2018-04-11 19:44

本文选题：阿尔茨海默病 + 重复经颅磁刺激　；参考：《生物医学工程研究》2017年04期

【摘要】：探讨重复经颅磁刺激(r TMS)对阿尔茨海默病(AD)大鼠学习记忆能力的影响,以及非线性动力学分析在大鼠脑电信号处理方面的应用。(1)将学习记忆无差异的60只SD大鼠随机分为(r TMS+AD)组、AD组和正常组,每组16只,多余的大鼠备用。(2)建立双侧海马区注射Aβ淀粉样蛋白(Aβ1-42)的AD大鼠模型,通过Morris水迷宫验证模型。(3)连续14 d对(r TMS+AD)组大鼠进行10 Hz的r TMS,AD组与正常组进行伪刺激。(4)利用水迷宫系统测试三组大鼠学习记忆能力,使用Cerebus 64通道神经电生理信号记录系统连续7 d采集三组大鼠头皮脑电。(5)在MATLAB中编程得到三组大鼠各个通道脑电信号能量谱以及LZ复杂度,利用SPSS软件对脑电信号和水迷宫数据进行统计分析。(1)AD组较正常组大鼠水迷宫逃避潜伏期变长,距离平台平均距离变远,差异有统计学意义(P0.05);(r TMS+AD)组较AD组大鼠水迷宫逃避潜伏期变短,距离平台平均距离减小,差异有统计学意义(P0.05)。(2)AD组较正常组大鼠脑电中低频(delta+theta)和高频(alpha+beta)的能量比值增高,部分通道差异无统计学意义(P0.05);(r TMS+AD)组大鼠较AD组大鼠的低频与高频的能量比值部分通道升高部分降低,只有左颞叶的差异有统计学意义(P0.05)。(3)(r TMS+AD)组较AD组大鼠各通道脑电LZ复杂度升高,AD组较正常组大鼠各通道脑电LZ复杂度降低,差异均有统计学意义(P0.05)。r TMS能改善AD大鼠的学习记忆能力以及增加其脑电信号的LZ复杂度,LZ复杂度较AR模型功率谱估计更适合客观的诊断AD并且评价r TMS对AD大鼠脑电信号的影响。
[Abstract]:To investigate the effects of repetitive transcranial magnetic stimulation (TMS) on learning and memory ability in rats with Alzheimer's disease (AD).60 SD rats with no difference in learning and memory were randomly divided into AD group (n = 16) and normal group (n = 16).The model of AD rats injected with A 尾 -amyloid protein A 尾 1-42) in bilateral hippocampal area was established.The Morris water maze model was used to test the learning and memory ability of the three groups by using the water maze system to test the learning and memory ability of the rats in the r TMS AD group (10 Hz) and the normal group (P < 0.05).Cerebus 64-channel neuroelectrophysiological signal recording system was used to collect scalp electroencephalogram (EEG) of three groups of rats for 7 days. The data were programmed in MATLAB to obtain the energy spectrum and LZ complexity of each channel EEG signal in three groups of rats.The data of EEG and water maze were statistically analyzed by SPSS software. The escape latency of water maze in AD group was longer than that in normal group, and the average distance from the platform became longer.Compared with AD group, the escape latency of water labyrinth was shorter and the average distance from the platform was decreased. The difference was statistically significant compared with the normal group. The energy ratios of the AD group were significantly higher than those of the normal group, and the energy ratios of the AD group were significantly higher than those of the normal group (P 0.05) and the high frequency group (P < 0.05), and the energy ratio of the AD group was significantly higher than that of the control group (P < 0.05).There was no significant difference in some channels between the two groups. The ratio of low frequency to high frequency of energy in the group of P0.05 TMS was higher than that of the group of AD, and the ratio of low frequency to high frequency was partly increased and partly decreased.The LZ complexity of each channel in AD group was significantly higher than that in AD group, and the LZ complexity of each channel in AD group was lower than that in normal group.The differences were statistically significant. P0.05. r TMS could improve the learning and memory ability of AD rats and increase the LZ complexity of EEG in AD rats. The LZ complexity was more suitable for the objective diagnosis of AD than the power spectrum estimation of AR model and the evaluation of r TMS for AD rats.The effect of EEG.
【作者单位】：涿州市医院;新乡医学院;
【基金】：河南省生物精神病重点实验室开放课题项目(ZDSYS2014005)
【分类号】：R749.16

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