自主跑轮运动对阿尔兹海默病模型小鼠认知、情绪以及杏仁核炎性因子表达的影响
发布时间：2018-04-26 20:59
本文选题：阿尔茨海默病 + 自主跑轮运动 ； 参考：《神经解剖学杂志》2017年06期
【摘要】：目的:探讨自主跑轮运动对阿尔茨海默病(Alzheimer’s disease,AD)模型小鼠空间记忆能力、情绪,以及小鼠脑内杏仁核NF-κB、TNF-α和IL-10表达的影响。方法:将18只3月龄的雄性昆明小鼠随机分为:二甲基亚砜(DMSO)对照组(n=6)和AD模型组(n=12)。AD模型组小鼠通过侧脑室内注射Aβ_(1-42)进行造模,造模一周后采用Y迷宫新异臂实验验证造模是否成功,并将造模成功的AD小鼠随机分成AD安静组(n=6)和AD运动组(n=6)。AD运动组进行为期6周的自主跑轮运动,AD安静组与DMSO对照组在同等环境下正常饲养6周。运动结束后,采用Y迷宫新异臂实验、高架十字迷宫实验、强迫游泳实验分别检测三组小鼠的空间记忆能力、焦虑、抑郁情绪,并统计分析。行为学实验结束后,用蛋白免疫印迹(Western Blot)实验检测三组小鼠杏仁核NF-κB、TNF-α和IL-10的表达变化。结果:(1)Y迷宫新异臂实验结果显示:造模一周后,AD模型组在新异臂中停留的时间(88.2 s±8.19 s)显著低于DMSO对照组(135.48 s±8.63 s,P㩳0.01);运动结束后,AD运动组在新异臂中停留的时间是AD安静组的1.7倍(P㩳0.01),但与DMSO对照组无统计学差异。(2)高架十字迷宫实验结果显示:AD运动组在开放臂中停留的时间较AD安静组显著升高(P㩳0.01),但与DMSO对照组无统计学差异。(3)强迫游泳实验结果显示:AD运动组的不动时间(88.64 s±5.23 s)明显低于AD安静组(236.13s±7.04 s,P㩳0.001),但与DMSO对照组无统计学差异。(4)蛋白免疫印迹结果显示:促炎因子TNF-α和NF-κB在AD安静组的表达均显著高于AD运动组和DMSO对照组(P㩳0.05),而抗炎因子IL-10的表达与之相反,低于AD运动组和DMSO组(P㩳0.05),各检测因子在AD运动组和DMSO组的表达无明显差异。结论:自主跑轮运动可有效改善AD模型小鼠的空间记忆以及焦虑抑郁情绪,并且改善杏仁核内炎性细胞因子的表达,从而发挥其脑保护作用。
[Abstract]:Objective: to investigate the effects of self-running wheel exercise on spatial memory ability, emotion and the expression of NF- 魏 B TNF- 伪 and IL-10 in the amygdala of Alzheimer's disease (AD) mice. Methods: eighteen 3-month-old male Kunming mice were randomly divided into two groups: dimethyl sulfoxide (DMSO) control group (n = 6) and AD model group (n = 12). After a week of modeling, the Y-maze new arm experiment was used to verify whether the model was successful or not. The successful AD mice were randomly divided into AD quiet group and AD exercise group for 6 weeks. The AD quiet group and the DMSO control group were fed in the same environment for 6 weeks. After exercise, Y maze new arms experiment, elevated cross maze test and forced swimming test were used to detect the spatial memory ability, anxiety and depression of the three groups. The expression of NF- 魏 B IL-10 and TNF- 伪 in the amygdala of the three groups were detected by Western blot assay after behavioral experiment. Results the results of the experiment showed that the retention time of AD model group was 88.2 s 卤8.19 s after the model was made, which was significantly lower than that of the DMSO control group (135.48 s 卤8.63 s), and that of AD exercise group was AD in the new arm after exercise. The results of the elevated cross maze test showed that the staying time in open arm of the control group was significantly higher than that in the quiet group, but there was no significant difference between the control group and the control group in DMSO. 3) obsessive-compulsion was observed in the control group (P < 0. 01, P < 0. 01, P < 0. 01, P < 0. 01), but no significant difference was found between the control group and the control group (P < 0. 01). The results of swimming test showed that the immobility time of exercise group was 88.64 s 卤5.23 s), which was significantly lower than that of control group (236.13 s 卤7.04 s), but there was no significant difference with DMSO control group. 4) the results of Western blot showed that the expression of TNF- 伪 and NF- 魏 B in AD quiet group was higher than that in control group. The expression of anti-inflammatory factor IL-10 was significantly higher than that of AD exercise group and DMSO control group. Compared with AD exercise group and DMSO group, there was no significant difference in the expression of each factor between AD exercise group and DMSO group. Conclusion: the self-running wheel exercise can effectively improve the spatial memory, anxiety and depression of AD mice, and improve the expression of inflammatory cytokines in the amygdala, so as to play a protective role in the brain.
【作者单位】： 山西医科大学人体解剖学教研室;
【基金】：山西医科大学科技创新基金(C01201002);山西医科大学基础医学院331基金(201213);山西医科大学大学生创新项目(2010121) 山西省高等学校科技项目(21021005)
【分类号】：R-332;R749.16
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本文编号：1807632
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