自主跑轮运动对阿尔茨海默病模型小鼠学习记忆能力和海马内炎性细胞因子表达的影响

发布时间：2018-04-27 22:43

本文选题：阿尔茨海默病 + 海马　；参考：《中国运动医学杂志》2017年04期

【摘要】：目的:探讨自主跑轮运动对阿尔茨海默病(AD)模型小鼠学习记忆能力和海马内肿瘤坏死因子-α(TNF-α)、白介素10(IL-10)、核转录因子κB(NF-κB)表达的影响。方法:将45只跑轮合格的3月龄雄性昆明小鼠随机分为二甲基亚砜(DMSO)对照组(n=15)和Aβ1-42寡聚体注射组(n=30),分别于侧脑室注射DMSO和Aβ1-42寡聚体,Aβ1-42寡聚体注射1周后两组小鼠进行Morris水迷宫实验,通过比较两组小鼠的逃避潜伏期和穿越平台次数,检测Aβ1-42寡聚体注射组与DMSO对照组小鼠的学习记忆能力,证实侧脑室注射Aβ1-42寡聚体能够有效模拟AD模型。之后将Aβ1-42寡聚体注射组小鼠随机分成AD运动组(n=15)和AD安静组(n=15),AD运动组进行为期6周的自主跑轮运动,AD安静组和DMSO对照组正常饲养6周,不运动。AD运动组跑轮结束后,3组小鼠采用Morris水迷宫实验,通过比较3组小鼠的逃避潜伏期和穿越平台次数,检测3组小鼠的学习记忆能力。运动后的3组小鼠均采用免疫荧光染色方法观察小鼠海马CA1区和CA3区TNF-α、IL-10、NF-κB阳性表达率变化。结果:(1)Morris水迷宫实验结果表明,侧脑室注射药物1周后,Aβ1-42寡聚体注射组小鼠的逃避潜伏期明显高于DMSO对照组(P0.05),穿越平台所在区域的次数显著低于DMSO对照组(P0.05);跑轮结束后,AD安静组小鼠的逃避潜伏期明显高于AD运动组和DMSO对照组(P0.05),穿越平台所在区域的次数明显低于AD运动组和DMSO对照组(P0.05)。(2)免疫荧光结果显示,NF-κB和促炎细胞因子TNF-α在海马CA1区和CA3区的表达趋势均一致,AD安静组的阳性表达率显著高于AD运动组和DMSO对照组(P0.05),而IL-10的阳性表达率则显著低于AD运动组和DMSO对照组(P0.05),各检测指标在AD运动组和DMSO对照组的阳性表达率无显著差异(P0.05)。结论:(1)自主跑轮运动可有效改善AD模型小鼠的学习记忆能力;(2)自主跑轮运动一方面通过抑制NF-KB的过度活化减少促炎细胞因子TNF-α的表达,另一方面促进抗炎细胞因子IL-10的表达。
[Abstract]:Aim: to investigate the effects of self-running wheel exercise on the learning and memory ability and the expression of tumor necrosis factor- 伪 (TNF- 伪), interleukin-10 (IL-10) and nuclear transcription factor 魏 B (NF- 魏 B) in the hippocampus of Alzheimer's disease (AD) mice. Methods: 45 male Kunming mice were randomly divided into two groups: control group (n = 15) and A 尾 1-42 oligomer injection group (n = 30). DMSO and A 尾 1-42 oligomer A 尾 1-42 were injected into the lateral ventricle for 1 week, respectively. Morris water maze experiment, By comparing the escape latency and the times of crossing the platform between the two groups, the learning and memory abilities of mice in the A 尾 1-42 oligomer injection group and the DMSO control group were measured. It was proved that the injection of A 尾 1-42 oligomer into the lateral ventricle could effectively simulate the AD model. Then the mice in the A 尾 1-42 oligomer injection group were randomly divided into AD exercise group (n = 15), AD quiet group (n = 15) and AD control group (n = 6). Morris water maze test was used to test the learning and memory ability of the three groups by comparing the escape latency and the times of crossing the platform. The positive expression rate of TNF- 伪 IL-10 and NF- 魏 B in the CA1 and CA3 regions of the hippocampus were observed by immunofluorescence staining in the three groups after exercise. Results the results of Morris water maze test showed that, The escape latency of mice in the A 尾 1-42 oligomer injection group was significantly higher than that in the DMSO control group, and the frequency of crossing the platform area was significantly lower than that in the DMSO control group, and the escape latency of the mice in the quiet group was significantly lower than that in the DMSO control group after the end of the running wheel. The expression of NF- 魏 B and TNF- 伪 in CA1 and CA3 regions of hippocampus was significantly higher than that of AD exercise group and DMSO control group, and the frequency of crossing the region of platform was significantly lower than that of AD exercise group and DMSO control group. The positive expression rate of IL-10 in AD quiet group was significantly higher than that in AD exercise group and DMSO control group, while the positive expression rate of IL-10 in AD exercise group and DMSO control group was significantly lower than that in AD exercise group and DMSO control group. There was no significant difference in the positive expression rate between AD exercise group and DMSO control group. Conclusion (1) Self-running wheel exercise can effectively improve the learning and memory ability of AD model mice. On the one hand, it can reduce the expression of pro-inflammatory cytokine TNF- 伪 by inhibiting the excessive activation of NF-KB, and on the other hand, it can promote the expression of anti-inflammatory cytokine IL-10.
【作者单位】：山西医科大学人体解剖学教研室;
【基金】：山西医科大学科技创新基金项目(C01201002);山西医科大学基础医学院331基金项目(201213);山西医科大学大学生创新项目(2010121) 山西省高等学校科技项目(21021005)
【分类号】：R749.16;R-332

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