老年性痴呆患者颞叶皮层差异基因的生物信息学分析

发布时间：2018-04-29 08:12

本文选题：老年性痴呆 + 颞叶皮层　；参考：《中国老年学杂志》2016年16期

【摘要】：目的探讨老年性痴呆患者颞叶皮层的生物学网络调控机制及关键节点在痴呆早期的病机机制。方法从基因芯片公共数据库GEO中下载老年性痴呆患者颞叶皮层的基因芯片数据,采用在线分析软件STRING和PANTHER对表达明显差异的基因进行生物信息学调控网络分析,寻找关键节点基因。结果在40个差异表达基因编码的蛋白中,有19个蛋白与其他蛋白存在相互作用关系,TBP、AKT1、CS、HSPB1及PARD6A蛋白为调控网络的关键节点蛋白。差异明显的基因主要涉及分子结合、催化活性及转录调节活性功能,与阿尔茨海默病(AD)-早老素信号通路、γ-氨基丁酸合成通路、AD-淀粉样蛋白分泌酶通路有关,参与了颞叶皮层的细胞间通讯、糖脂代谢、细胞转运等生理学过程。结论 AD颞叶皮层的发病与TBP、AKT1、CS、HSPB1及PARD6A等基因密切相关,主要涉及AD-早老素信号通路、γ-氨基丁酸合成通路和AD-淀粉样蛋白分泌酶通路,有利于针对性的采用具有调节相应网络功能的药物进行早期干预,保护AD患者的认知功能。
[Abstract]:Objective to investigate the biological network regulation mechanism of temporal cortex in patients with Alzheimer's disease (AD) and the pathogenesis of key nodes in the early stage of dementia. Methods the microarray data of temporal cortex of Alzheimer's disease patients were downloaded from the common database of gene chip GEO. The bioinformatics control network analysis of the genes with obvious difference was performed by STRING and PANTHER. Search for key node genes. Results among the 40 proteins encoded by differentially expressed genes, 19 proteins interacted with other proteins. HSPB1 and PARD6A proteins were the key node proteins in the regulatory network. The differentially expressed genes are mainly involved in molecular binding, catalytic activity and transcriptional regulatory activity, and are related to the AD-early aging signal pathway of Alzheimer's disease, the 纬 -aminobutyric acid synthesis pathway and the AD-amyloid secretase pathway. Involved in the temporal cortex intercellular communication, glucose metabolism, cell transport and other physiological processes. Conclusion the pathogenesis of AD temporal cortex is closely related to the genes such as HSPB1 and PARD6A, which are mainly involved in AD-presentin signaling pathway, gamma-aminobutyric acid synthesis pathway and AD-amyloid secretase pathway. It is beneficial to the early intervention of drugs with the function of regulating the corresponding network and protecting the cognitive function of AD patients.
【作者单位】：中国中医科学院中医临床基础医学研究所;河南省省直第二医院肿瘤科;北京师范大学认知神经科学与学习国家重点实验室;南方医科大学中医药学院;
【基金】：国家自然科学基金资助项目(81274001) 国家科技重大专项—重大新药创制(2013ZX09103002-002,2013ZX09301307)
【分类号】：R749.16

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