前列腺素E1上调血管性痴呆大鼠海马组织VEGF、BDNF的表达

发布时间：2018-04-30 15:12

本文选题：前列腺素E + 血管性痴呆　；参考：《中国神经精神疾病杂志》2015年08期

【摘要】：目的探讨前列腺素E1(prostaglandin E1,PGE1)对双侧颈总动脉永久性结扎(bilateral common carotid artery occlusion,BCCAO)大鼠学习记忆功能及海马VEGF、BDNF表达的影响。方法 48只大鼠随机分为PGE1治疗组(PGE1,10μg·kg-1·d-1,iv)、PGE1+VEGFR拮抗剂组(PGE1,10μg·kg-1·d-1,iv;SU5416,25 mg·kg-1·d-1,ip)、溶剂对照组、假手术组,每组12只。术后15 d进行认知功能障碍筛选实验,24 d给予相应药物连续注射7d,药物处理后2 d予Morris水迷宫实验评估大鼠学习记忆功能后处死,蛋白印迹法观察大鼠海马组织VEGF、BDNF的表达。结果与溶剂对照组及PGE1+VEGFR拮抗剂组比,PGE1治疗组平均潜伏期降低(P0.05),穿越次数增多(5.77±0.83 vs.2.88±0.47 vs.2.63±0.44,P0.01),且原平台象限停留时间百分比增大(32.28%±4.56%vs.20.42%±5.50%vs.23.08%±5.06%,P0.05);与溶剂对照组、假手术组相比,PGE1治疗增加了海马VEGF表达量(0.057±0.005 vs.0.038±0.002 vs.0.027±0.002,P0.05)、BDNF表达量(0.481±0.049 vs.0.339±0.021 vs.0.224±0.04,P0.05);与PGE1+VEGFR拮抗剂组比,PGE1治疗组VEGF表达量差异不明显(0.057±0.005 vs.0.053±0.003,P0.05),但BDNF表达量增多(0.481±0.049 vs.0.373±0.034,P0.05)。结论 PGE1可上调血管性痴呆大鼠海马组织VEGF、BDNF的表达并可改善血管性痴呆大鼠学习记忆功能,而VEGFR拮抗剂则可部分拮抗PGE1的这一作用。
[Abstract]:Objective to investigate the effects of prostaglandin E _ 1 (PGE _ 1) on learning and memory function and expression of VEGFNF-BDNF in hippocampus of bilateral common carotid artery occlusion (BCCAO) rats with permanent ligation of bilateral common carotid artery. Methods Forty-eight rats were randomly divided into PGE1 treatment group (n = 10) and control group (n = 12, sham operation group, n = 12). On the 15th day after operation, the cognitive dysfunction screening test was carried out and the corresponding drugs were injected continuously for 7 days. The rats were killed 2 days after treatment with Morris water maze test. The expression of VEGFBDNF in hippocampal tissue of rats was observed by Western blot. Results compared with the solvent control group and the PGE1 VEGFR antagonist group, the average latency of PGE1 group was significantly lower than that of the PGE1 group, and the crossing times increased by 5.77 卤0.83 卤0.47 vs.2.63 卤0.4 vs.2.63 卤0.01g, and the percentage of residence time in the original platform quadrant increased by 32.28% 卤4.56vs.20.42% 卤5.50vs.23.08% 卤5.06v. Compared with the control group, the expression of VEGF was increased by 0.057 卤0.005 卤0.002 vs.0.027 卤0.002 vs.0.027 in the sham operation group, and 0.481 卤0.049 vs.0.339 卤0.021 vs.0.224 卤0.04 vs.0.224 卤0.05 in the control group, but there was no significant difference in VEGF expression in the PGE1 treatment group compared with the PGE1 VEGFR antagonist group, but the increase in BDNF expression was 0.481 卤0.049 vs.0.373 卤0.034 P0.05a. Conclusion PGE1 can up-regulate the expression of VEGF and BDNF in hippocampus of vascular dementia rats and improve the learning and memory function of vascular dementia rats, while VEGFR antagonist can partly antagonize this effect of PGE1.
【作者单位】：暨南大学附属第四医院;广州市红十字会医院神经内科;广州市红十字会医院创伤外科研究所;
【基金】：广州市科技计划项目基金(编号:2014J4100077)
【分类号】：R749.13

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