COMT（Vall58Met）基因多态性、生活事件与重性抑郁障碍的相关性研究

发布时间：2018-05-04 16:19

本文选题：重性抑郁障碍 + COMT基因多态性　；参考：《天津医科大学》2012年硕士论文

【摘要】：目的：从分子生物学水平探讨儿茶酚胺氧位甲基转移酶(COMT)基因多态性(Val158Met)与重性抑郁障碍的发病、症状及严重程度的关系,分析COMT基因型和生活事件交互作用对抑郁严重程度的影响,进一步研究抑郁障碍的发病机制。方法：采用病例对照的研究方法,严格控制入组标准,按照DSM-IV的诊断标准,选取250例重性抑郁障碍患者和与之性别、年龄相匹配的250例正常对照作为研究对象。量表选用HAMD-17评定抑郁障碍的严重程度,而对病例组的生活事件评定采用生活事件量表(LES)。实验中抽取被试外周静脉血,提取基因组DNA,应用限制性片段长度多态性-聚合酶链反应(RFLP-PCR)技术对COMT Val158Met进行基因分型。运用SPSS19.0统计软件包进行数据分析,P0.05为有统计学差异。结果： 1、COMTVal158Met有三种基因型Val/Val, Val/Met和Met/Met。病例组和对照组中各基因型分布频率与期望频率的差别没有统计学差异,符合Hardy-Weinberg定律。 2、病例组和对照组中COMTVa1158Met各基因型及等位基因频率分布具有显著性差异(P0.05)；而病例组和对照组中,女性的基因型和等位基因分布有统计学差异,而在男性中的分布没有统计学差异(P0.05)。 3、GA基因型相对于GG基因型而言是患抑郁症的保护性因素,等位基因A相对G也是保护性因素。在女性中有同样的关联,但在男性中并未发现相似的关联。 4、不同基因型患者的HAMD总分、迟滞因子、Maier因子、核心因子的分值中具有显著性差异(P0.05),GA基因型与其他基因型在HAMD,总分、迟滞因子、核心因子间都有显著性差异(P0.05)。HAMD各条目中仅有“迟缓”存在统计学差异。 5、生活事件是抑郁严重程度的危险因素,基因和生活事件的交互作用对疾病的严重程度有显著影响(P=0.037)。结论： 1、COMTVal158Met多态性可能与中国天津地区人群重性抑郁障碍的发病有关,在女性中尤为明显。 2、COMT Val158Met多态性可能与抑郁症的严重程度、核心症状、迟滞症状、Maier症状相关,GA型基因可能与抑郁障碍的严重程度和某些表型有关系。 3、生活事件对抑郁障碍严重程度有影响；COMT Val158Met多态性和生活事件两者存在交互作用,对疾病的严重程度有显著影响。
[Abstract]:Objective: To explore the relationship between the polymorphism of catecholamine oxomethyltransferase gene (Val158Meta) and the onset, symptoms and severity of severe depressive disorder from the molecular biological level, and to analyze the effect of interaction of COMT genotype and life events on the severity of depression. To further study the pathogenesis of depression disorder. Methods: According to the diagnostic criteria of DSM-IV, 250 cases of patients with severe depressive disorder, sex and age matched with normal control were selected as the study objects. HAMD-17 was used to assess the severity of depressive disorder, while the life event scale was used to assess the life events in the case group. Genomic DNA was extracted from peripheral venous blood of the subjects, and COMT Val158Met genotyping was performed by restriction fragment length polymorphism (RFLP-PCR) technique. Using SPSS19.0 statistical software package for data analysis, P0.05 for statistical differences. Results: 1. COMTVal158Met has three genotypes: Valr / Val, Val/Met and Metr / Met. There was no significant difference in the frequency of genotype distribution and expected frequency between the case group and the control group, which was in accordance with Hardy-Weinberg 's law. 2There was significant difference in the frequency distribution of COMTVa1158Met genotypes and alleles between the case group and the control group (P 0.05), while the distribution of the genotype and allele in the case group and the control group was statistically different, but there was no significant difference in the distribution of the genotype and allele in the male (P 0.05). 3the GA genotype was a protective factor for depression compared with the GG genotype, and the allele A relative to G was also a protective factor. The same association was found in women, but not in men. 4. There were significant differences in total score of HAMD, hysteresis factor and factor of Maier in patients with different genotypes. There was significant difference in the scores of core factors between GA genotype and other genotypes in HAMD, total score, hysteresis factor. There were significant differences among the core factors. 5. Life events are the risk factors of depression, and the interaction of gene and life events has a significant effect on the severity of the disease. Conclusion: 1 the COMTVal158Met polymorphism may be associated with the onset of major depressive disorder in the population in Tianjin, China, especially in women. 2COMT Val158Met polymorphism may be associated with the severity of depression, core symptoms, and hysteretic symptoms, and the GA gene may be associated with the severity of depression and some phenotypes. 3. Life events had an effect on the severity of depressive disorder. There was interaction between COMT Val158Met polymorphism and life events, which had a significant effect on the severity of the disease.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.41

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