水迷宫训练对抑郁大鼠齿状回神经干细胞增殖和血管内皮生长因子表达的影响

发布时间：2018-05-05 23:54

本文选题：抑郁症 + 学习记忆　；参考：《中国医科大学学报》2014年01期

【摘要】：目的研究抑郁症学习记忆障碍与齿状回神经干细胞增殖和血管内皮生长因子(VEGF)表达的关系。方法 20只雌性Wistar大鼠随机分为对照组和抑郁症模型组(模型组)。复制慢性不可预见性应激模型。旷场实验评价大鼠行为学变化,Morris水迷宫定位航行实验评估大鼠学习记忆能力,电镜观察大鼠海马结构及其微小血管形态学变化,免疫组化技术检测Nestin及VEGF表达。结果与对照组比较,模型组大鼠的行为学水平和垂直活动得分显著降低;定位航行实验逃避潜伏时和游泳距离显著延长;海马微小血管口径变大,管壁变厚,内皮细胞结构不规则;Nestin免疫阳性细胞数和VEGF表达均明显低于对照组。结论抑郁症学习记忆障碍与慢性应激导致的海马微小血管损伤、海马齿状回神经再生抑制有关。
[Abstract]:Objective to study the relationship between learning and memory disorder and proliferation of neural stem cells and expression of vascular endothelial growth factor (VEGF) in dentate gyrus in depression. Methods Twenty female Wistar rats were randomly divided into control group and depression model group (model group). Copy chronic unpredictable stress model. Open field experiment to evaluate the behavioral changes in rats and Morris water maze navigation experiment to evaluate the learning and memory ability of rats, the morphological changes of hippocampal structure and small vessels in rats were observed by electron microscope, and the expressions of Nestin and VEGF were detected by immunohistochemical technique. Results compared with the control group, the scores of behavioral level and vertical activity in the model group were significantly decreased, the distance between escape latency and swimming was significantly prolonged, the diameter of the small vessels in the hippocampus became larger and the wall became thicker. The number of nestin immunoreactive cells and the expression of VEGF in endothelial cells with irregular structure were significantly lower than those in the control group. Conclusion the learning and memory disorder of depression is related to the damage of hippocampal microvessels and the inhibition of nerve regeneration in the dentate gyrus of the sea horse induced by chronic stress.
【作者单位】：沈阳体育学院生理学教研室;
【分类号】：R749.4

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