创伤后应激障碍样症状大鼠症状学特征与端粒和胰岛素样生长因子的相关研究

发布时间：2018-05-07 10:10

本文选题：创伤后应激障碍 + 端粒长度　；参考：《石河子大学》2016年硕士论文

【摘要】：第一部分经SPS创伤后PTSD样大鼠端粒和端粒结合蛋白的表达研究目的探讨创伤后应激障碍(post-traumatic stress disorder, PTSD)样症状大鼠外周血白细胞端粒长度的变化及海马CA1区端粒结合蛋白的表达。方法取成年健康雄性Wistar大鼠42只,建立大鼠PTSD模型,应用实时荧光定量PCR技术测量相对白细胞端粒长度；免疫荧光技术检测PTSD大鼠海马CA1区TRF1和TRF2的表达变化。结果经SPS刺激后大鼠端粒长度缩短速率较对照组加快,有统计学差异(P0.05)。TRF1和TRF2蛋白的表达均随着PTSD的发生表达增高,且在第14天表达最高,与对照组的表达有统计学差异(P0.05)。结论PTSD大鼠外周血白细胞端粒长度缩短速率加快及TRF1和TRF2在海马区表达增高,这与PTSD的病理发展和预后不良可能密切相关。第二部分经SPS创伤后PTSD样大鼠海马CA1区IGF-1及其受体的表达研究目的研究胰岛素样生长因子1(IGF-1)及其受体(IGF-1 receptor)在创伤后应激障碍(post-traumatic stress disorder, PTSD)大鼠的海马CA1区的表达。方法取成年健康雄性Wistar大鼠30只,采用国际认定的SPS方法刺激建立大鼠PTSD模型,应用免疫组化技术检测PTSD大鼠海马CA1区IGF-1和IGF-1 receptor的表达变化。结果经SPS刺激后大鼠海马CA1区IGF-1蛋白的表达随着PTSD的发生表达增高,且在第14天表达最高,与正常对照组的表达有统计学差异(P0.05)。而IGF-1 receptor蛋白的表达在应激前和应激后的第1天、第7天其表达程度未见明显差异(P0.05),而在第14天和第28天表达较前有所降低(P0.05)。结论应激后IGF-1的表达增高,但其受体的表达却没有同步增高,甚至有所降低,这可能在一定程度上限制了IGF-1的积极作用,这可能参与了PTSD的认知功能改变的病理生理学过程。
[Abstract]:Part one: expression of telomere and telomere binding protein in PTSD like rats after SPS trauma objective to investigate the changes of telomere length in peripheral white blood leukocytes and the expression of telomere binding protein in CA1 region of hippocampus in rats with post-traumatic stress disorder, PTSD) like symptoms after traumatic stress disorder. Methods 42 adult male Wistar rats were used to establish PTSD model. The telomere length of relative leukocytes was measured by real-time fluorescence quantitative PCR and the expression of TRF1 and TRF2 in the CA1 region of hippocampus of PTSD rats were detected by immunofluorescence technique. Results the shortening rate of telomere length of rats stimulated by SPS was faster than that of the control group. The expression of P0.05, TRF1 and TRF2 protein increased with the development of PTSD, and the highest expression was found on the 14th day, and there was a significant difference between the expression of TRF1 and the control group (P 0.05). Conclusion the increase of telomere length shortening rate and the expression of TRF1 and TRF2 in hippocampus of PTSD rats may be closely related to the pathological development and poor prognosis of PTSD. Part two: expression of IGF-1 and its receptors in the CA1 region of hippocampus of PTSD like rats after SPS trauma objective to study the expression of insulin-like growth factor (IGF-1) and its receptor (IGF-1 receptor) in the hippocampal CA1 region of post-traumatic stress disorder, PTSD) rats with post-traumatic stress disorder (PTSD). Methods the PTSD model was established in 30 adult healthy male Wistar rats stimulated by the internationally recognized SPS method. The expression of IGF-1 and IGF-1 receptor in the CA1 region of the hippocampus of PTSD rats was detected by immunohistochemical technique. Results after stimulation with SPS, the expression of IGF-1 protein in the CA1 area of hippocampus increased with the occurrence of PTSD, and the expression of IGF-1 protein was the highest on the 14th day, which was significantly different from that of the normal control group (P 0.05). However, there was no significant difference in the expression of IGF-1 receptor protein before and on the first day and the seventh day after stress, but on the 14th and 28th days, the expression of IGF-1 receptor protein was lower than that of the former. Conclusion the expression of IGF-1 increases after stress, but the expression of its receptor does not increase or even decrease, which may limit the positive effect of IGF-1 to some extent, which may be involved in the pathophysiological process of PTSD cognitive function change.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R749.5

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