慢性应激对大鼠行为学及脑神经颗粒素水平的影响及药物防治

发布时间：2018-05-12 02:15

本文选题：慢性不可预见性应激 + 学习记忆　；参考：《中风与神经疾病杂志》2016年04期

【摘要】：目的探讨慢性应激对大鼠外显行为学变化的影响,检测体内儿茶酚胺类物质及神经颗粒素(Neurogranin,Ng)表达的变化及三七皂苷Rg1的防治效果。方法成年雄性SD大鼠36只,随机分为对照组(CON)、模型组(CUS)、和治疗组(CUS-G),采用慢性不可预见性应激方法建立慢性应激动物模型;采用旷场试验检测行为学变化,运用Morris水迷宫实验进行学习记忆力测试,使用放射免疫法检测血浆、脑组织儿茶酚胺类含量,Western blot法检测皮质、海马、下丘脑Ng含量。结果 6 w慢性应激后,实验组大鼠在水平运动和垂直运动方面得分均明显低于对照组与药物组,均P0.05;水迷宫实验显示慢性应激后动物逃避潜伏期明显延长,而治疗组大鼠逃避潜伏期呈下降趋势(P0.05);血浆、脑组织去甲肾上腺素与多巴胺在对照组、实验组与药物组间无明显变化;慢性应激大鼠皮质、海马、下丘脑Ng含量水平皆与模型大鼠有差异(P0.05、P0.01、P0.05),治疗大鼠皮质、海马、下丘脑Ng含量亦皆与模型大鼠有差异(P0.01、P0.05、P0.05)。结论慢性应激研究领域,神经颗粒素的敏感性优于单胺类神经递质,可作为敏感指标加以观察;100 mg/kg剂量的三七皂苷Rg1能够抑制应激所致学习记忆功能紊乱,对应激所致外显行为有积极的调节作用,对脑内神经颗粒素表达水平降低趋势有较好抑制作用。
[Abstract]:Objective to investigate the effect of chronic stress on the behavior of rats in vitro, to detect the expression of catecholamines and neurogranin in vivo and the preventive effect of panax notoginsenoside Rg1. Methods Thirty-six adult male Sprague-Dawley rats were randomly divided into control group, model group and treatment group. Chronic unpredictable stress was used to establish chronic stress animal model, open field test was used to detect behavioral changes. Morris water maze test was used to test learning and memory. Plasma and brain catecholamines were measured by radioimmunoassay. Ng contents in cortex, hippocampus and hypothalamus were detected by Western blot method. Results after 6 weeks of chronic stress, the scores of horizontal and vertical movement in the experimental group were significantly lower than those in the control group and the drug group (P0.05.The water maze test showed that the escape latency was significantly prolonged after chronic stress. In the treatment group, the escape latency of rats decreased (P 0.05), the plasma and brain tissues of norepinephrine and dopamine did not change in the control group, the experimental group and the drug group showed no significant change; the cortex, hippocampus, hippocampus of chronic stress rats, The content of Ng in hypothalamus was different from that in model rats. The contents of Ng in cortex, hippocampus and hypothalamus were also different from those in model rats. Conclusion in the research field of chronic stress, the sensitivity of neurogranulins is better than that of monoamine neurotransmitters, and it can be used as a sensitive index to observe the inhibitory effect of panax notoginsenoside Rg1 on learning and memory disorder induced by stress. It has a positive regulation on explicit behavior induced by stress and a good inhibitory effect on the decreasing trend of neurogranin expression in the brain.
【作者单位】：昆明医科大学科研实验中心;昆明医科大学基础医学院人体解剖学与组织胚胎学系;昆明医科大学基础医学院实验中心;
【基金】：2012年云南省教育厅科学研究基金(2012C005) 2014年国家自然科学基金(81460192)
【分类号】：R749.5

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