番茄红素对原代小鼠皮质神经元的保护作用及机制研究

发布时间：2018-05-15 00:16

本文选题：番茄红素 + 脑皮质神经元　；参考：《中国病理生理杂志》2017年02期

【摘要】：目的:观察番茄红素(lycopene)对氧化损伤的原代皮质神经元的保护效应并探讨其作用机制。方法:采用原代细胞培养技术体外分离培养小鼠皮质神经元,通过免疫荧光染色法检测微管相关蛋白2(microtubuleassociated protein 2,MAP-2)的表达进行鉴定。将神经元分为4组:正常神经元组、叔丁基过氧化氢(tert-butyl hydroperoxide,t-BHP)处理组、t-BHP+lycopene处理组和lycopene处理组,培养24 h,采用MTT法检测各组神经元的活力;采用流式细胞技术检测各组神经元内ROS的水平;Western blot法检测各组神经元Bax、Bcl-2、caspase-3、cleaved caspase-3及细胞色素C蛋白表达的变化。结果:Lycopene能明显提高t-BHP处理的神经元活性,降低tBHP处理的神经元内ROS含量,同时上调Bcl-2蛋白的表达,降低Bax、cleaved caspase-3和细胞色素C蛋白的表达(P0.05)。结论:Lycopene能够对抗t-BHP诱导的原代皮质神经元的损伤,抑制神经元凋亡,其机制可能与降低神经元内ROS的水平及上调的表达有关。
[Abstract]:Aim: to investigate the protective effect of lycopene on oxidative injury of primary cortical neurons and its mechanism. Methods: mouse cortical neurons were isolated and cultured by primary cell culture technique, and the expression of microtubule-associated protein (2(microtubuleassociated protein _ 2 / MAP-2) was detected by immunofluorescence staining. The neurons were divided into four groups: normal neuron group, tert-butyl hydroperoxidet-BHPtreated group, t-BHP lycopene group and lycopene treatment group, and cultured for 24 hours. The activity of neurons in each group was detected by MTT method. The level of ROS in neurons of each group was detected by flow cytometry and the expression of caspase-3 and cytochrome C protein in neurons of each group were detected by Western blot. Results the activity of neurons treated with t-BHP was significantly increased, the content of ROS in neurons treated with tBHP was decreased, the expression of Bcl-2 protein was upregulated, and the expression of caspase-3 and cytochrome C protein was decreased by weight Lycopene. ConclusionLycopene can antagonize the injury of primary cortical neurons induced by t-BHP and inhibit the apoptosis of neurons. The mechanism may be related to the decrease of the level of ROS and the up-regulation of the expression of ROS in neurons.
【作者单位】：暨南大学医学院病理生理学系脑科学研究所国家中医药管理局三级科研实验室;
【基金】：国家自然科学基金资助项目(No.81471236)
【分类号】：R749.16

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