Morris水迷宫训练对老年痴呆大鼠模型学习记忆能力及海马组织肿瘤坏死因子-α、超氧化物歧化酶、丙二醛水平的影响

发布时间：2018-05-15 22:40

本文选题：Morris水迷宫训练 + 痴呆　；参考：《中国老年学杂志》2017年08期

【摘要】：目的观察Morris水迷宫训练对老年痴呆(AD)大鼠学习记忆能力及海马组织肿瘤坏死因子(TNF)-α、超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法随机将32只SD雄性大鼠分为假手术组、模型组、Morris水迷宫短期训练组和Morris水迷宫长期训练组;以穹隆-海马伞切断建立痴呆大鼠模型;运用Morris水迷宫进行训练;采用跳台实验以及水迷宫实验测试大鼠的学习和记忆能力;采用酶联免疫吸附法(ELISA)测定大鼠海马组织TNF-α的水平,比色法检测大鼠海马组织SOD活性和MDA含量。结果 Morris水迷宫训练后AD大鼠学习记忆能力显著提高(P0.01);大鼠海马区TNF-α、MDA含量明显降低(P0.05)、SOD含量明显升高(P0.05)。结论 Morris水迷宫训练能明显改善大鼠的学习记忆能力,其机制可能与提高海马区SOD含量、降低TNF-α和MDA水平有关,而且水迷宫训练时间越长,恢复越明显。
[Abstract]:Objective to observe the effects of Morris water maze training on learning and memory ability, tumor necrosis factor TNF- 伪, superoxide dismutase (SOD) and malondialdehyde (MDA) in hippocampus of senile dementia rats. Methods Thirty-two Sprague-Dawley male rats were randomly divided into sham-operation group and Morris water maze training group and Morris water maze model was established by fornix-hippocampal umbrella transection, and Morris water maze was used for training. The level of TNF- 伪 in rat hippocampal tissue was measured by enzyme linked immunosorbent assay (Elisa), and the activity of SOD and the content of MDA in hippocampal tissue were measured by colorimetry. Results the learning and memory ability of AD rats was significantly increased after Morris water maze training, and the content of TNF- 伪 -MDA in hippocampus was significantly decreased in AD rats. Conclusion Morris water maze training can obviously improve the learning and memory ability of rats. The mechanism may be related to the increase of SOD content in hippocampus and the decrease of TNF- 伪 and MDA levels. The longer the training time of water maze is, the more obvious the recovery is.
【作者单位】：湖北民族学院医学院;湖北民族学院医学院病理解剖学教研室;
【基金】：国家自然科学基金资助项目(No.81360654)
【分类号】：R749.16;R-332

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