Znf804a基因在发育期鼠脑神经元的迁移、增殖及分化中的功能

发布时间：2018-05-18 07:38

本文选题：脑发育 + 神经元迁移　；参考：《中国心理卫生杂志》2017年11期

【摘要】：目的:以小鼠为模型研究精神分裂症易感基因Znf804a在神经发育中的功能。方法:利用胚胎电转技术,将Znf804a的shRNA(shZnf804a)质粒及其对照质粒(pSUPER)电转进入E14.5的癌症研究所(ICR)小鼠脑室区,每种质粒电转3只,分别为敲低(knock down)Znf804a组和对照组,比较两组神经元在皮层板(CP)区的比例来评价神经元的迁移速度;比较两组神经元前体细胞形成神经球的直径来评估神经元增殖速度;比较体外培养神经元前体细胞中Nestin染色阳性神经元的比例检测神经元分化速度。结果:敲低Znf804a小鼠的神经元迁移到CP区的比例比对照组低(11.8%vs.75.4%,P0.001);神经元前体细胞形成神经球的直径比对照组大(295μm vs.172μm,P0.01);神经元前体细胞中,Nestin染色阳性的神经元比例比对照组高(31.5%vs.9.6%,P0.01)。结论:敲低Znf804a小鼠神经元迁移速度和分化速度比对照组神经元慢,增殖速度比对照组神经元快,提示Znf804a在小鼠脑发育过程中发挥重要功能。
[Abstract]:Objective: to study the function of susceptibility gene Znf804a in nervous development in mice. Methods: the shRNA-shZnf804a) plasmid of Znf804a and its control plasmid pSUPERA were electrotransferred into the ventricular region of the mice of cancer research institute of E14.5 by electroporation technique. Each plasmid was electrotransferred into 3 mice, which were knock down knock down)Znf804a group and control group, respectively. The migration rate of neurons was evaluated by comparing the proportion of neurons in the cortex (CPV) region, the diameter of neuronal precursor cells forming neurospheres to evaluate the proliferation rate of neurons in the two groups. The rate of Nestin staining positive neurons in cultured neurons in vitro was compared. Results: the percentage of neurons migrating to CP area in Znf804a knockout mice was 11.8vs.75.4i P0.001, the diameter of neuronal precursor cells was larger than that of control group (295 渭 m vs.172 渭 m P0.01), and the percentage of nestin positive neurons in neurons precursor cells was higher than that in control group (31.5 vs.9.6m P0.01). Conclusion: the migration and differentiation of neurons in Znf804a knockout mice are slower than those in control group, and the proliferation rate is faster than that in control group, suggesting that Znf804a plays an important role in the development of mouse brain.
【作者单位】：北京大学第六医院北京大学精神卫生研究所国家精神心理疾病临床医学研究中心(北京大学第六医院)卫生部精神卫生学重点实验室(北京大学);
【分类号】：R749.3

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