改良方法制备的血管性痴呆模型大鼠的学习记忆能力表现

发布时间：2018-05-20 21:15

本文选题：血管性痴呆 + 模型　；参考：《中国药理学与毒理学杂志》2014年03期

【摘要】：目的探讨改良方法制备的血管性痴呆(VD)模型的成模可信程度。方法采用间隔3 d分2次结扎大鼠双侧颈总动脉法(改良2-VO)与左侧切口、不分离翼腭动脉的大鼠大脑中动脉梗死/再灌注法(改良MCAO/R)制备VD模型,Morris水迷宫分别进行定位航行实验及空间探索实验,通过逃避潜伏期、目标区域穿越次数及搜索策略测定各组大鼠的学习记忆能力,HE染色光学显微镜下观察大鼠海马组织CA1区组织结构的变化。结果术后20 d,与假手术组比较,两模型组的大鼠逃避潜伏期均延长(P0.05),尤以改良2-VO组的逃避潜伏期最长;至术后41 d时改良MCAO/R组与假手术组无显著性差异(P0.05),而改良2-VO组与假手术组始终具显著性差异(P0.01),且此时两模型组间比较差异也显著(P0.01)。空间探索实验,与假手术组比较,两模型组分别穿越平台次数均减少,尤以改良2-VO组最为突出;至术后第42天改良MCAO/R组与假手术组已无显著性差异(P0.05)。且改良MCAO/R组术后第20天与第41天的潜伏期及术后第21天与第42天的穿越平台次数比较,也均具有显著性差异(P0.01)。搜索策略结果显示,改良2-VO组游泳轨迹多为边缘式和随机式,改良MCAO/R组通过训练的增多搜索策略大部分改变为趋向式与直线式。两模型组大鼠海马CA1区组织结构均出现细胞数目减少,细胞排列紊乱稀疏,细胞形态不完整,结构不正常,胞浆稀少,胞核与胞浆界限模糊,细胞核深染,固缩呈三角形或不规则形,核仁不明显,并出现增生胶质细胞的现象。其中改良2-VO组最为严重,改良MCAO/R组较轻。结论两种改良方法制作的VD模型均可导致大鼠出现行为学及病理学的改变,其中改良2-VO模型更适用于较长时期的药物疗效研究,操作简便,且模型稳定。而改良MCAO/R模型是较为理想的短期药效研究或预防药物疗效研究的造模方法之一。
[Abstract]:Objective to investigate the reliability of VD-based vascular dementia model. Methods the bilateral common carotid artery (2-VOO) was ligated twice and left incision was performed on the left side of the rat by using the method of bilateral common carotid artery ligation (2-VOO) at intervals of 3 days. The middle cerebral artery infarction / reperfusion method (modified MCAO / R) was used to prepare VD model of VD rats by Morris water maze, and the location navigation experiment and space exploration experiment were carried out respectively. The learning and memory abilities of the rats in each group were measured. The changes of the structure of the CA1 region in the hippocampus of the rats were observed under HE staining optical microscope. Results 20 days after operation, compared with sham operation group, the escape latency of rats in the two model groups was prolonged (P 0.05), especially in the modified 2-VO group. There was no significant difference between the modified MCAO/R group and the sham operation group at the end of 41 days after operation (P 0.05), but there was a significant difference between the modified 2-VO group and the sham operation group (P 0.01), and the difference between the two model groups was also significant (P 0.01). Compared with the sham-operated group, the number of traversing the platform was decreased in the two model groups, especially in the modified 2-VO group, but there was no significant difference between the modified MCAO/R group and the sham-operation group on the 42nd day after operation (P 0.05). In the modified MCAO/R group, there were significant differences in the latency between the 20th day and the 41st day, and the times of crossing the platform on the 21st day and the 42nd day after operation, and there was also significant difference between the two groups (P 0.01). The results of search strategy showed that the swimming trajectory of modified 2-VO group was mostly marginal and random, and the increased search strategy of modified MCAO/R group changed to trend and straight line by training. In both groups, the number of cells decreased, the cells were disordered and sparse, the morphology of the cells was incomplete, the structure was abnormal, the cytoplasm was rare, the boundary between cytoplasm and nucleus was blurred, and the nucleus was deeply stained. Pyknosis is triangular or irregular, nucleolus is not obvious, and the phenomenon of proliferation of glial cells. The modified 2-VO group was the most serious, and the modified MCAO/R group was mild. Conclusion the VD model made by the two improved methods can cause behavioral and pathological changes in rats. The modified 2-VO model is more suitable for the study of drug efficacy in a long period of time, and the model is stable and easy to operate. The modified MCAO/R model is one of the ideal methods for short-term pharmacodynamics research or prophylactic efficacy study.
【作者单位】：石河子大学药学院教育部新疆特种植物药资源重点实验室;
【基金】：国家自然科学基金(30860334);国家自然科学基金(30560171)~~
【分类号】：R749.16

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