卒中后抑郁大鼠学习记忆障碍研究

发布时间：2018-05-21 06:34

本文选题：卒中 + 应激　；参考：《浙江大学》2012年硕士论文

【摘要】：目的对比脑缺血与慢性应激所致学习记忆障碍及海马病变的强弱,为临床改善脑卒中后抑郁(Post-stroke depression PSD)提供参考。实验方法 40只成年雄性SD大鼠平均分为4组：对照组、抑郁(应激)组、卒中(缺血)组与PSD(缺血加应激)组,卒中处理采用改良的选择性大脑中动脉栓塞术；抑郁处理采用连续3周的慢性不可预见性温和应激；Morris水迷宫实验评价依赖海马的学习记忆功能；免疫组织化学染色及半定量RT-PCR观察海马CA3区脑源性神经营养因子(Brain-derive neurotrophic factor, BDNF)的表达变化。结果缺血或应激均可使大鼠学习功能明显下降,表现为与同时点对照组比较,逃避潜伏期显著延长,二者的综合作用更明显。慢性应激对学习功能的影响强于脑缺血损伤。缺血或抑郁减弱记忆功能,但二者的作用差异无统计学意义。与对照相比,缺血显著增强海马CA3区BDNF的表达(27.0±2.5与20.1±2.1),应激降低BDNF的表达(15.2±1.8与20.1±2.1),二者综合作用仍显著降低BDNF的表达(8.2±1.5),差异均具有统计学意义(,=52.87,P0.05)。结论缺血与慢性应激均降低大鼠学习记忆功能,应激对认知功能的损害高于缺血,而应激与缺血的综合作用对学习记忆损害与抑制BDNF表达作用更明显,提示进行PSD的综合治疗时,更重视心理社会应激干预和抑郁状态的改善。
[Abstract]:Purpose The learning and memory disorders and hippocampal lesions induced by cerebral ischemia and chronic stress were compared to provide a reference for clinical improvement of post-stroke depression (Post-stroke depression PSDs). Experimental method Forty adult male SD rats were divided into four groups on average: control group, depression group, stroke group and PSD group. The treatment of stroke was treated by modified selective middle cerebral artery embolization. Depression was treated with chronic unpredictable mild stress and Morris water maze for 3 weeks in order to evaluate the learning and memory function of the hippocampus. Immunohistochemical staining and semi-quantitative RT-PCR were used to observe the expression of brain-derived neurotrophic factor, BDNF) in hippocampal CA3. Result Both ischemia and stress could significantly decrease the learning function of rats. Compared with the control group at the same time, the escape latency was significantly prolonged, and the combined effect of the two was more obvious. The effect of chronic stress on learning function is stronger than that on cerebral ischemia. Ischemia or depression weakened memory function, but there was no significant difference between them. Compared with the control group, ischemia significantly enhanced the expression of BDNF in hippocampal CA3 area (27.0 卤2.5,20.1 卤2.1), and decreased the expression of BDNF by 15.2 卤1.8 and 20.1 卤2.1. The combined effect of ischemia and ischemia still significantly decreased the expression of BDNF (8.2 卤1.5), and the difference was statistically significant (P 0.05). Conclusion Both ischemic and chronic stress decreased the learning and memory function of rats, and the damage to cognitive function of rats was higher than that of ischemia, while the combined effect of stress and ischemia on learning and memory impairment and inhibition of BDNF expression was more obvious. More attention was paid to psycho-social stress intervention and improvement of depression.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.1

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