雌激素对去卵巢大鼠抑郁样行为及杏仁核NGF表达的影响

发布时间：2018-05-23 22:45

本文选题：雌二醇 + 神经生长因子　；参考：《中国医科大学学报》2016年12期

【摘要】：目的通过观察雌二醇治疗对雌激素剥夺大鼠的抑郁样行为及杏仁核神经生长因子(NGF)含量的变化,探讨雌二醇治疗抑郁症的作用靶点及作用机制。方法健康成年雌性SD大鼠随机分为假手术组(SHAM组,n=10)、卵巢切除组(OVX组,n=10)及去卵巢雌二醇治疗组(OVX+E组,n=10)3组。雌二醇治疗8周后通过悬尾及糖水偏爱实验观察雌二醇对雌激素剥夺大鼠行为学的影响;随后用免疫组织化学方法测量和分析杏仁核NGF神经元的数量及面积。结果与SHAM组大鼠比较,OVX组大鼠糖水偏爱实验中糖水偏爱率显著下降(P0.01)、悬尾实验中不动时间延长(P0.01),血清雌二醇水平及杏仁核NGF表达均显著下降(P均0.01);8周雌二醇治疗改善了去卵巢大鼠的抑郁样行为,与OVX组大鼠比较,血清雌激素水平及杏仁核NGF表达水平均显著增加(P均0.01)。结论雌二醇治疗改善了去卵巢大鼠的抑郁样行为,可能与雌二醇增加去卵巢大鼠的血清雌激素水平及增强杏仁核NGF的表达有关。
[Abstract]:Objective to investigate the effects of estradiol on depression and the changes of nerve growth factor (NGF) content in amygdaloid nucleus of estrogen deprived rats. Methods healthy adult female Sprague-Dawley rats were randomly divided into three groups: sham-operated group (sham group), ovariectomized group (OVX group) and ovariectomized estradiol group (OVX E group). After 8 weeks of estradiol treatment, the effects of estradiol on the behavior of estrogen deprived rats were observed by tail suspension and sugar water preference experiments, and the number and area of NGF neurons in amygdala were measured and analyzed by immunohistochemical method. Results compared with SHAM group, the rate of glucose water preference was significantly decreased in OVX group, and the immobility time was prolonged in tail suspension test. The serum estradiol level and NGF expression in amygdala were significantly decreased in OVX group after 8 weeks of estradiol treatment. Improved depressive behavior in ovariectomized rats, Compared with OVX group, the level of serum estrogen and the expression of NGF in amygdala increased significantly (P < 0.01). Conclusion Estradiol therapy can improve the depressive behavior of ovariectomized rats, which may be related to the increase of serum estrogen level and the increase of amygdaloid NGF expression in ovariectomized rats.
【作者单位】：山西医科大学汾阳学院护理学系;济宁市荣复军人医院精神科;四川省广安市精神卫生中心精神科;山西医科大学汾阳学院解剖教研室;中北大学体育学院运动医学教研室;
【分类号】：R749.4

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