自主跑轮运动对慢性不可预见性轻度应激大鼠抑郁样行为及神经内分泌因子近日节律的影响

发布时间：2018-05-24 03:20

本文选题：自主跑轮运动 + 慢性不可预见性轻度应激　；参考：《中国病理生理杂志》2017年10期

【摘要】：目的:探讨8周自主跑轮运动对慢性不可预见性轻度应激(CUMS)大鼠抑郁样行为及外周神经内分泌因子生物节律表达的影响,从时间生物学方面探讨运动抗抑郁、促进脑康复的作用机制。方法:雄性SD大鼠90只,随机分为对照(control)组、模型(model)组及模型运动(model+exercise)组。采用CUMS方式建立抑郁模型,模型运动组前5周自愿跑轮,从第6周开始,附加21 d慢性复合应激,模型组于同一时点造模21 d。采用糖水偏好实验、旷场行为及高架十字迷宫系统观测大鼠焦虑情绪的行为学变化。行为学检测结束后第2天,连续24 h分6个时点(ZT1、ZT5、ZT9、ZT13、ZT17、ZT21)处死动物取血,每个时点处死5只大鼠,放射免疫法测定上述6个时点血清促肾上腺皮质激素(ACTH)含量,ELISA检测6个相同时点血清皮质酮(CORT)、褪黑素(MT)及血管活性肠肽(VIP)的含量,采用单一余弦法拟合曲线比较慢性应激大鼠各指标的节律周期、振幅、峰相位、中值的变化特点以及运动对其的影响。结果:与对照组相比,21 d的CUMS使模型组大鼠体重增加量明显减少(P0.01),糖水消耗量明显降低(P0.01);旷场实验中爬格数、直立次数及中央格停留时间均显著下降,而粪粒数明显增多(P0.01);高架十字迷宫中开放臂停留时间的百分比和开放臂进入次数的百分比明显降低(P0.01)。运动对CUMS诱导的大鼠抑郁样行为有明显的改善作用。血浆近日节律输出肽及相关激素方面,慢性应激至抑郁样行为充分表达后,模型组大鼠血浆ACTH、CORT的相位与对照组完全相反,表现为时相的大幅度提前、含量波动幅度减小、昼夜分泌节律紊乱;MT的24 h分泌节律完全丧失且整体水平下降,表达量显著低于对照组;VIP虽仍存在24 h节律,但振幅明显低于对照组,峰相位也延迟6 h,表达量显著高于对照组。8周跑轮运动能显著改善应激大鼠的抑郁样行为及激素分泌量的异常,使血浆ACTH、MT、CORT和VIP的近日节律恢复正常。结论:运动可拮抗CUMS致大鼠的抑郁样行为,其作用机制可能与其在一定程度上能够调整外周神经内分泌激素的昼夜节律性和改变激素的分泌量有关。
[Abstract]:Objective: to investigate the effects of 8-week independent wheel running on depressive behavior and the expression of peripheral neuroendocrine factors in rats with chronic unpredictable mild stress (CUMS), and to explore the antidepressant effects of exercise on chronobiology. The mechanism of promoting brain rehabilitation. Methods: 90 male Sprague-Dawley rats were randomly divided into control group, model group and exercise model group. The depression model was established by CUMS. The model group ran the wheel voluntarily in the first 5 weeks. From the 6th week, the model group was induced by chronic compound stress for 21 days, and the model group was made at the same time point for 21 days. The behavioral changes of anxiety in rats were observed by sugar water preference test, open field behavior and elevated cross maze system. On the second day after the end of behavioral examination, the animals were sacrificed at 6 time points (ZT1, ZT1, ZT5, ZT13, ZT17, ZT21) and 5 rats were killed at each time point. Serum ACTH levels were measured by radioimmunoassay (RIA) and serum corticosterone (Cort), melatonin (MTT) and vasoactive intestinal peptide (VIP) were detected by Elisa at the same time points. Single cosine method was used to fit the curve to compare the rhythm period, amplitude, peak and phase of each index of chronic stress rats, as well as the change characteristics of median value and the effect of exercise on it. Results: compared with the control group, CUMS for 21 days significantly reduced the body weight gain and the consumption of sugar water in the model group, and significantly decreased the number of creeping cells, the number of upright cells and the residence time of the central grid in the open field experiment. The percentage of open arm retention time and the number of open arm entry times in the elevated cross maze decreased significantly. Exercise can significantly improve the depressive behavior induced by CUMS in rats. In plasma circadian output peptide and related hormones, the phase of plasma ACTHCORT in the model group was opposite to that in the control group after the chronic stress to depression-like behavior was fully expressed. The 24 h secretion rhythm of MT was completely lost and the whole level decreased, and the expression of VIP was significantly lower than that of the control group, although there was still 24 h rhythm, but the amplitude was significantly lower than that of the control group. The peak phase was also delayed for 6 hours, and the expression level was significantly higher than that in the control group, and the depressive behavior and abnormal hormone secretion of stress rats were significantly improved, and the circadian rhythm of plasma ACTH / MTCORT and VIP returned to normal. Conclusion: exercise can antagonize the depressive behavior induced by CUMS in rats. The mechanism may be related to the regulation of circadian rhythm of peripheral neuroendocrine hormones and the change of hormone secretion.
【作者单位】：成都体育学院运动医学与健康学院;
【基金】：四川省教育厅重点科研项目(No.13za0074);四川省教育厅创新团队项目(No.13TD0010) 国家体育总局运动医学重点实验室暨运动医学四川省重点实验室基金资助项目(No.2014CTYY005) 成都体育学院博士基金资助项目(No.15YJBS05);成都体育学院科研骨干出国进修计划(No.2016-2017)
【分类号】：R749.4

【相似文献】