miRNA-195在小鼠早发精神分裂症发病机制中的作用

发布时间：2018-05-31 01:28

本文选题：miRNA- + 精神分裂症　；参考：《中国老年学杂志》2017年14期

【摘要】：目的探究miRNA-195在小鼠早发精神分裂症发病机制中的作用。方法通过LNA-miR195抑制内源性miR-195,Morris水迷宫观测小鼠行为学的变化。给小鼠注射10、20和40 mg/kg氯胺酮,检测miRNA-195的蛋白表达变化以及行为变化。结果 LNA-miR195能够高效抑制内源性miR-195的表达,miR-195的表达被抑制后小鼠在Morris水迷宫中的潜伏期延长,记忆检测时穿越平台位置的次数明显减少,72 h时从电跳台平台上跳下的时间显著降低(P0.05)。不同浓度氯胺酮处理的小鼠miR-195蛋白表达上调,显著影响小鼠体重的增加,雄性和雌性小鼠在Morris水迷宫的空间探索实验期没有明显的差异,在电跳台阶段40 mg/kg处理的雄性小鼠和20 mg/kg和40 mg/kg处理组的雌性大鼠与盐水对照组比较差异具有统计学意义(P0.05)。结论 miRNA-195在早发精神分裂症发病中具有重要的作用。
[Abstract]:Objective to explore the role of miRNA-195 in the pathogenesis of early onset schizophrenia in mice. Methods the behavior of mice was observed by LNA-miR195 inhibiting endogenous miR-195 Morris water maze. Mice were injected with ketamine for 10 ~ 20 and 40 mg/kg to detect the changes of miRNA-195 protein expression and behavior. Results LNA-miR195 could effectively inhibit the expression of endogenous miR-195. The latency of Morris water labyrinth in mice was prolonged after the inhibition of the expression of miR-195. The number of times of passing through the platform position during memory detection was significantly reduced when the time of jumping from the platform was significantly decreased after 72 h. The expression of miR-195 protein was up-regulated in mice treated with different concentrations of ketamine, which significantly affected the weight gain of mice. There was no significant difference between male and female mice in the spatial exploration of Morris water maze. The male mice treated with 40 mg/kg and the female rats treated with 20 mg/kg and 40 mg/kg were significantly different from the saline control group in the electric jumper stage (P 0.05). Conclusion miRNA-195 plays an important role in the early onset of schizophrenia.
【作者单位】：武汉市精神卫生中心司法鉴定科;武汉市中医医院医务处;武汉市中医医院院办;武汉市精神卫生中心综合科;
【基金】：湖南省自然科学基金(2015JJ1010)
【分类号】：R749.3

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