不同光源对抑郁模型大鼠行为学及褪黑素影响探讨

发布时间：2018-06-01 10:54

本文选题：生物节律光源 + 抑郁模型　；参考：《中国职业医学》2016年01期

【摘要】：目的观察节能灯、白炽灯和生物节律光源对抑郁模型大鼠行为学的影响,初步探讨其对褪黑素的影响机制。方法无特定病原体级健康雌性6周龄SD大鼠40只,适应性饲养后,随机取30只于11~14周龄时采用双侧卵巢切除联合孤养、慢性不可预知温和应激的复合式方法建立抑郁模型后,随机分为节能灯、白炽灯和生物节律灯干预组,每组10只,于17周龄时分别予相应的光源光照干预,为期3周;另10只大鼠为对照组,常规饲养。在17、19、20和21周龄时称量大鼠体质量,在7、14、20周龄时分别采用糖水偏好实验和旷场实验对大鼠进行行为学测试,在21周龄时测定大鼠1个暗周期(19:30)至明周期(08:30)7个时间点的周围血褪黑素水平,每组每个时间点随机测定1只大鼠。结果光照干预前的17周龄时,4组大鼠体质量差异无统计学意义(P0.05);光照干预后,3个干预组大鼠体质量在17~20周龄均呈随时间增加而增长趋势(P0.05);21周龄时4组大鼠体质量分别与同组20周龄比较,差异均无统计学意义(P0.05)。7周龄时,4组大鼠糖水消耗量和站立评分差异均无统计学意义(P0.05);造模后未予光照干预前的14周龄时,3个干预组大鼠糖水消耗量和站立评分均低于对照组(P0.05),3个干预组大鼠上述2个指标差异均无统计学意义(P0.05);光照干预结束后20周龄时,4组大鼠糖水消耗量和站立评分差异均无统计学意义(P0.05)。3个干预组大鼠周围血褪黑素高峰值均高于对照组;节能灯和节律灯干预组大鼠周围血褪黑素高峰分别较对照组提前或延后2 h出现,而白炽灯干预组与对照组大鼠周围血褪黑素高峰出现时间相近。结论生物节律灯与节能灯、白炽灯对抑郁模型大鼠行为均有改善作用,3种光照干预效果相近;生物节律灯干预可延迟周围血褪黑素高峰出现时间。
[Abstract]:Objective to observe the effects of energy saving lamp, incandescent lamp and biological rhythm light source on behavior of rats with depression and to explore the mechanism of their effects on melatonin. Methods 40 healthy female SD rats of 6 weeks old without specific pathogens were selected randomly after adaptive feeding. 30 SD rats were randomly selected at the age of 1114 weeks to establish depression model by bilateral ovariectomies combined with solitary feeding and chronic unpredictable mild stress. The rats were randomly divided into three groups: energy saving lamp, incandescent lamp and biological rhythm lamp intervention group, 10 rats in each group were treated with corresponding light source for 3 weeks at the age of 17 weeks, the other 10 rats were as control group and fed routinely. The body weight of the rats was measured at 20 and 21 weeks of age, and the behavior of the rats was measured by sugar water preference test and open field test at the age of 70.14 and 20 weeks, respectively. The peripheral blood melatonin levels were measured at 21 weeks of age from 1 dark cycle (19: 30) to 08: 30 (7 time points). One rat was randomly measured at each time point in each group. Results there was no significant difference in body mass between the 4 groups at the age of 17 weeks before exposure to light, while the body weight of the rats in the three intervention groups increased with time at the age of 17 to 20 weeks, while the body weight of the 4 groups increased with the age of 21 weeks. 20 weeks of age were compared with those of the same group. There was no significant difference in sugar water consumption and standing score between the four groups at the age of P0.05.7 weeks, and there was no significant difference in the consumption of sugar water and the standing score of the rats in the three intervention groups at the age of 14 weeks before the exposure to light after the model was established, and there was no significant difference in the consumption of sugar water and the standing score of the rats in the three intervention groups at the age of 14 weeks before the model was established. Compared with the control group, there was no significant difference in the above two indexes among the three intervention groups, but there was no significant difference in sugar water consumption and standing score between the four groups at the age of 20 weeks after the light treatment. The rats in the three intervention groups had no significant difference in week weeks. The peak value of melatonin in peripheral blood was higher than that in control group. The peak of melatonin in peripheral blood of rats in the intervention group of energy saving lamp and rhythm lamp was earlier or delayed by 2 hours than that in the control group, but the time of the peak appeared in the incandescent lamp group and the control group was similar. Conclusion the effects of biological rhythm lamp, energy saving lamp and incandescent lamp on behavior of depressive rats were similar, and the intervention of biological rhythm lamp could delay the peak time of melatonin in peripheral blood.
【作者单位】：北京大学第六医院北京大学精神卫生研究所卫生部精神卫生学重点实验室(北京大学) 国家精神心理疾病临床医学研究中心(北京大学第六医院);北京大学物理学院北京大学宽禁带半导体研究中心;北京大学基础医学院;广东省职业病防治院广东省职业病防治重点实验室;广东省深圳市龙华新区疾病预防控制中心;北京大学公共卫生学院;北京大学第三医院;北京大学实验动物科学部;北京大学生命科学学院;
【基金】：国家高技术研究发展计划(863计划,2011AA03A113)
【分类号】：R749.4;R-332

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