CYP27B1基因多态性与精神分裂症发病及代谢指标的相关性

发布时间：2018-06-01 16:38

本文选题：CYP27B1 + 基因多态性　；参考：《中南大学》2013年硕士论文

【摘要】：目的：本研究主要目的是探讨CYP27B1基因多态性与精神分裂症发病的关联,以及与利培酮治疗后引起的代谢指标变化是否有关。方法：入组222例精神分裂症住院患者及150例健康志愿者,采用聚合酶连接检测反应技术(polymerase chain reaction-ligase detection reaction, PCR-LDR)检测rs10877012和rs4646536基因型。采用德国西门子拜耳ADVIA Centaur自动化学发光免疫分析仪直接测定胰岛素(insulin, Ins);采用柏林格曼姆日立7170自动生化分析仪检测血脂四项(TC、TG、HDLC. LDLC)。数据采用SPSS16.0软件分析。结果：采用χ2检验分析得到所有基因型符合Hardy-Weinberg平衡,rs10877012和rs4646536基因多态性与精神分裂症发病无统计学意义(P0.05)。利培酮治疗4周后胰岛素与TG较0周有统计学意义(P0.05), TC、HDLC、LDLC无统计学意义,P0.05。采用方差分析得到rs10877012基因型与0周(P=0.012)及服药4周胰岛素变化值(p=0.019)有统计学意义,rs4646536基因型与0周(P=0.013)及4周胰岛素变化值(P=0.022)有统计学意义。结论：CYP27B1rs10877012和rs4646536两个基因多态性和精神分裂症的发病无统计学意义,说明CYP27B1基因多态性与中国中南地区汉族精神分裂症发病无关。精神分裂症患者用利培酮单药治疗4周后胰岛素、TG水平较0周水平显著升高。rs10877012基因型与利培酮治疗后胰岛素变化有关,携带T等位基因的患者胰岛素更易升高。rs4646536基因型与0周及服用利培酮4周胰岛素变化有关,携带C等位基因的患者服药治疗后胰岛素更易升高,说明CYP27B1基因多态性可能与利培酮治疗后胰岛素耐受有关。图10幅,表17个,参考文献44篇。
[Abstract]:Objective: to investigate the association between CYP27B1 gene polymorphism and schizophrenia and whether it is related to metabolic changes after risperidone treatment. Methods: rs10877012 and rs4646536 genotypes were detected by polymerase chain reaction-ligase detection reaction (PCR-LDR) in 222 inpatients with schizophrenia and 150 healthy volunteers. Insulin and insulin were directly determined by Siemens Bayer ADVIA Centaur automatic chemiluminescence immunoassay (ADVIA Centaur), and the serum lipids were determined by Berlin Gumman Hitachi 7170 automatic biochemical analyzer. LDLC. The data was analyzed by SPSS16.0 software. Results: 蠂 2 analysis showed that all genotypes were consistent with Hardy-Weinberg balance rs10877012 and rs4646536 gene polymorphisms, and there was no significant difference between the polymorphism of rs10877012 and the incidence of schizophrenia (P 0.05). After 4 weeks of risperidone treatment, there was significant difference in insulin and TG between 0 week and 0 week (P 0.05), but there was no significant difference in LDLC between TCX HDLC and TG (P 0.05). The analysis of variance showed that there were significant differences between the genotype of rs10877012 and 0 week (P 0.012) and the change value of insulin at 4 weeks (P 0.019). There was statistical significance between genotype rs4646536 and 0 week (P < 0.013) and the change value of insulin at 4 weeks (P = 0.022). Conclusion there is no significant difference between the two gene polymorphisms of CYP27B1rs10877012 and rs4646536 and the incidence of schizophrenia, indicating that the polymorphism of CYP27B1 gene is not related to the onset of schizophrenia in the Han nationality in the central and southern regions of China. Insulin TG level in schizophrenia patients was significantly higher than that at 0 weeks after risperidone treatment for 4 weeks. Genotype rs10877012 was associated with insulin changes after risperidone treatment. Insulin of patients with T allele was more likely to increase. Rs4646536 genotype was associated with insulin changes at 0 weeks and risperidone at 4 weeks, and insulin was more likely to increase in patients with C allele after treatment with risperidone. The results suggest that CYP27B1 gene polymorphism may be associated with insulin resistance after risperidone therapy. Ten figures, 17 tables, 44 references.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.3

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