SH-SY5Y细胞p38 MAPK信号转导通路对α7尼古丁受体蛋白水平的影响

发布时间：2018-06-07 07:15

本文选题：阿尔茨海默病 + α　；参考：《中国老年学杂志》2017年05期

【摘要】：目的研究p38 MAPK信号传导通路激动及抑制对SH-SY5Y细胞α7神经型乙酰胆碱受体(n AChR)蛋白水平的影响并探讨受体蛋白与p38通路之间的关系。方法分别用p38 MAPK激活剂Anisomycin和p38 MAPK阻断剂SB203580激动和阻断SH-SY5Y细胞p38 MAPK通路蛋白的活化及其表达,Western印迹方法检测α7 n AChR蛋白水平。结果细胞经Anisomycin处理后,p38 MAPK蛋白表达不变,p-p38 MAPK(Thr180/Tyr182)蛋白水平升高了71%(P0.01),同时细胞α7 n AChR蛋白表达升高了80%(P0.01).细胞经SB203580处理后,p38 MAPK蛋白表达不变,p-p38 MAPK(Thr180/Tyr182)蛋白水平降低了62%(P0.01),提示p38 MAPK信号通路被抑制,同时细胞α7 n AChR蛋白表达降低了80%(P0.01)。结论 Anisomycin能激动SH-SY5Y细胞p38 MAPK信号转导通路,引起α7 n AChR蛋白表达明显增强;SB203580能阻断SH-SY5Y细胞p38 MAPK信号转导通路,引起α7 n AChR蛋白水平显著降低。
[Abstract]:Objective to investigate the effect of activation and inhibition of p38 MAPK signal transduction pathway on 伪 7 neuronal acetylcholine receptor (伪 7) acetylcholine receptor n (AChR) protein in SH-SY5Y cells and to explore the relationship between receptor protein and p38 pathway. Methods p38 MAPK activator Anisomycin and p38 MAPK blocker SB203580 were used to stimulate and block the activation of p38 MAPK pathway protein and its expression in SH-SY5Y cells. The level of 伪 7 n AChR protein was detected by Western blot. Results after treated with Anisomycin, the expression of p38 MAPK protein was invariant and the protein level of p-p38 MAPKnThr180 / Tyr182) was increased, while the expression of 伪 7n AChR protein was increased by 80% P0.01a. After treated with SB203580, the expression of p38 MAPK protein was invariant and the protein level of p-p38 MAPKnThr180 / Tyr182) decreased, suggesting that the p38 MAPK signaling pathway was inhibited, and the expression of 伪 7n AChR protein decreased by 80% P0.01a. Conclusion Anisomycin can stimulate the p38 MAPK signal transduction pathway in SH-SY5Y cells, and increase the expression of 伪 7n AChR protein. SB203580 can block the p38 MAPK signal transduction pathway in SH-SY5Y cells and decrease the level of 伪 7 n AChR protein.
【作者单位】：贵州医科大学贵州省医学分子生物学重点实验室;贵州医科大学地方病与少数民族疾病教育部重点实验室;
【基金】：国家自然科学基金资助项目(81360178) 贵州省教育厅“2011协同创新中心”(黔教合协同创新中心[2014]06) 贵州省科技厅重大专项(黔科合重大专项字2014[6008]);贵州省科技厅项目(黔科合SY字[2013]3020;黔科合LH字[2016]7365
【分类号】：R749.16

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