辛伐他汀对脂多糖诱导的小鼠抑郁行为及神经炎症的影响

发布时间：2018-06-09 02:45

本文选题：辛伐他汀 + 抑郁症　；参考：《中国药理学通报》2017年03期

【摘要】：目的探讨辛伐他汀对脂多糖(LPS)诱导小鼠抑郁行为的影响及其机制。方法给予ICR小鼠单剂量腹腔注射脂多糖0.5 mg·kg~(-1)建立抑郁小鼠模型。在给予小鼠LPS前每天灌胃给予辛伐他汀,连续给药1周。采用强迫游泳实验、悬尾试验、糖水偏好度实验检测小鼠抑郁样行为;采用Western blot检测小鼠前额叶皮层NF-κB p65、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)蛋白水平。结果悬尾试验和强迫游泳实验结果显示,与正常对照组相比,LPS组小鼠不动时间明显延长,给予辛伐他汀10和20 mg·kg~(-1)能明显减少抑郁小鼠不动时间;糖水偏好度检测结果显示,与正常对照组相比,LPS组小鼠糖水偏好度明显降低,而给予辛伐他汀10和20 mg·kg~(-1)能增加抑郁小鼠糖水偏好度;Western blot结果显示,LPS组小鼠前额叶皮层NF-κB p65,IL-1β和TNF-α明显升高,给予辛伐他汀10和20 mg·kg~(-1)能抑制抑郁小鼠炎性蛋白表达升高。结论辛伐他汀能够改善LPS诱导的小鼠抑郁样行为和神经炎症。
[Abstract]:Objective to investigate the effect and mechanism of simvastatin on depression induced by lipopolysaccharide (LPS) in mice. Methods ICR mice were given a single intraperitoneal injection of lipopolysaccharide (0.5 mg 路kg ~ (-1) to establish a model of depression. Simvastatin was administered daily for 1 week before LPS was given to mice. The depressive behavior of mice was detected by forced swimming test, tail suspension test and sugar water preference test, and the protein levels of NF- 魏 B p65, interleukin-1 尾 -IL-1 尾, tumor necrosis factor- 伪 TNF- 伪 in the prefrontal cortex of mice were detected by Western blot. Results the results of tail suspension test and forced swimming test showed that the immobility time of lipopolysaccharide group was significantly longer than that of normal control group, and simvastatin 10 and 20 mg 路kg ~ (-1) could significantly reduce the immobility time of depression mice, and the results of sugar water preference test showed that the immobility time of depression mice was significantly decreased after administration of simvastatin 10 and 20 mg 路kg ~ (-1). Compared with the normal control group, the sugar water preference of lipopolysaccharide group was significantly decreased, while simvastatin 10 and 20 mg / kg 路kg -1) could increase the glucose water preference of depression mice. The results showed that the prefrontal cortex NF- 魏 B p65, IL-1 尾 and TNF- 伪 were significantly increased in LPS group. Simvastatin (10 and 20 mg 路kg ~ (-1) inhibited the increase of inflammatory protein expression in depressive mice. Conclusion Simvastatin can improve the depressive behavior and neuroinflammation induced by LPS in mice.
【作者单位】：温州医科大学附属第一医院药学部;温州市龙湾区第一人民医院药剂科;
【基金】：浙江省自然科学基金资助项目(No LY16H30005)
【分类号】：R749.4

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